Product Name:
WNK2-3
Product Number:
ab-nk253-3
Target Full Name: Serine/threonine-protein kinase WNK2
Target Alias: Antigen NY-CO-43; KIAA1760; NY-CO-43; P,OKcl.13; P/OKcl.13; PRKWNK2; Protein kinase with no lysine 2; Protein kinase, lysine-deficient 2; WNK lysine deficient protein kinase 2; SDCCAG43; KAIA302; ENSG00000165238
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK253-3
Antibody Target Type: Pan-specific
Protein UniProt: Q9Y3S1
Protein SigNET: Q9Y3S1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human WNK2 sequence peptide Cat. No.: PE-01AZD90
Antibody Immunogen Sequence: CGSKTASFAASDPVRS
Antibody Immunogen Description: Corresponds to amino acid residues G2283 to S2297; C-terminus
Production Method: Corresponds to amino acid residues G2283 to S2297; C-terminus
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 250-300 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human WNK1.
Related Product 1: WNK2-3 blocking peptide
Related Product 2: WNK2-1 pan-specific antibody (Cat. No.: AB-NK253-1)
Related Product 3: WNK2-2 pan-specific antibody (Cat. No.: AB-NK253-2)
Scientific Background: WNK2 (PRKWNK2) is a protein-serine/threonine kinase of the Other group and WNK family. It plays a critical role in the regulation of electrolyte homeostasis, signalling survival, and proliferation. Activation requires autophosphorylation of S356. Phosphorylation of S352 also promotes increased phosphotransferase activity. It activates sodiu-coupled chloride cotransporters and inhibits potassium-coupled chloride cotransporters. It is also involved in reducing ERK1/2 activation. ERK1/2 expression can also increase through activation of of MEK1 via Rac1-mediate PAK1 activation. WNK2 may be a tumour suppressor protein (TSP). WNK2 is also called serologically defined colon cancer antigen 43. Loss of WNK2 expression was found in adult gliomas and triggers Rac1-mediated tumour cell invasiveness. Loss of expression of WNK2 is also found in meningioma and facilitates increased cell cycle progression and proliferation.
