Product Name:
SIK-1
Product Number:
ab-nk251-1
Target Full Name: Salt-inducible serine/threonine-protein kinase SIK1; Serine-threonine-protein kinase SNF1-like kinase 1
Target Alias: Msk; Myocardial SNF1-like kinase; Salt-inducible kinase; Salt-inducible kinase 1; Serine/threonine-protein kinase SNF1-like kinase 1; Serine/threonine-protein kinase SNF1LK; SIK1; SN1L1; SNF1-like kinase; SNF1LK
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK251-1
Antibody Target Type: Pan-specific
Protein UniProt: P57059
Protein SigNET: P57059
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SIK (SNF1LK) sequence peptide Cat. No.: PE-01AYV99
Antibody Immunogen Sequence: MVIMSEFSADPAGQGC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to G15; N-terminus
Production Method: Corresponds to amino acid residues M1 to G15; N-terminus
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-85 kDa.
Antibody Specificity: Very high
Related Product 1: SIK-1 blocking peptide
Related Product 2: SIK-2 pan-specific antibody (Cat. No.: AB-NK251-2)
Related Product 3: SIK-3 pan-specific antibody (Cat. No.: AB-NK251-3)
Related Product 4: SIK-pT182 phosphosite-specific antibody (Cat. No.: AB-PK812)
Related Product 5: AMPK/SIK KinSub - AMPK/SIK peptide substrate AMARAASAAALARRR
Scientific Background: SIK (SNF1LK) is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It can mediate gluconeogenesis, lipogenesis, cell cycle regulation, muscle differentiation, muscle growth, and tumour suppression. Substrates of SIK can include SREBF1, HDAC5, PPME1, TORC1/CRTC1, HDAC4, and TORC2/CRTC2. Phosphorylation at S186 increases phosphotransferase activity and induces interaction with 14-3-3. It is also activated by phosphorylation on T182 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. SIK may be a tumour requiring protein (TRP). The loss of SIK1 has been correlated with poor prognosis for individuals suffering from breast cancer. Kinase phosphotransferase activity can be decreased without affecting autophosphorylation levels of SIK through either a S135A, or a S209A, or a S248A mutation. LKB1/LKB1-mediated phosphorylation and activation of SIK can be inhibited through a T182A mutation in SIK. Partial inhibition of phosphorylation and of kinase phosphotransferase activity has been observed with the S186A/D/C/G mutation. Abrogation of autophosphorylation and kinase phosphotransferase activity can occur with a S186T mutation.