Product Name:
Obscn-2
Product Number:
ab-nk247-2
Target Full Name: Obscurin-myosin light chain kinase
Target Alias: Obscurin; UNC89; ARHGEF30; Q96AA2; ENSG00000154358
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK247-2
Antibody Target Type: Pan-specific
Protein UniProt: Q5VST9
Protein SigNET: Q5VST9
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Obscn sequence peptide Cat. No.: PE-01AXY85
Antibody Immunogen Sequence: CLSHPWFLKSMPAEEA
Antibody Immunogen Description: Corresponds to amino acid residues C6715 to A6730; Kinase last alpha-chain
Production Method: Corresponds to amino acid residues C6715 to A6730; Kinase last alpha-chain
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 36.6-45 kDa.
Related Product 1: Obscn-2 blocking peptide
Related Product 2: Obscn-1 pan-specific antibody (Cat. No.: AB-NK247-1)
Related Product 3: Obscn-3 pan-specific antibody (Cat. No.: AB-NK247-3)
Scientific Background: Obscn is a protein-serine/threonine kinase of the CAMK group and Trio family. It is a protein kinase, and a cytoskeletal calmodulin and titin-interacting RhoGEF. It is part of a family of giant sacromeric signalling proteins that includes titin and nebulin, and has been implicated in the organization of myofibrils during assembly and mediation of interactions between the the sarcoplasmic reticulum and myofibrils. It seems to be an adapter protein that is involved in assembly of myosin into sarcomeric A bands in striated muscle. Isoform 3 together with ANK1 isoform Mu17/Ank1.5 may provide a molecular link between the sarcoplasmic reticulum and myofibrils. Although it demonstrates a high rate of mutation in cancer cells, it is unclear how this implicates in oncogenesis. A chromosomal aberration involving Obscn has been reported in Wilms tumour, and the gene has a translocation t(1;7)(q42;p15) with PTHB1. Due to its large size, there is a greater chance of mutations being detected in this protein in human cancers, and these may be mainly bystander mutations.