Product Name:
FGFR4-4
Product Number:
ab-nk239-3
Target Full Name: Fibroblast growth factor receptor 4
Target Alias: CD334; JTK2; MGC20292; MPK11; TKF; CCDS4411.1; ENSG00000160867
Product Type Specific: FGFR4 protein kinase pan-specific antibody
Antibody Code: NK239-3
Antibody Target Type: Pan-specific
Protein UniProt: P22455
Protein SigNET: FGFR4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human FGFR4 sequence peptide Cat. No.: PE-01BBW99
Antibody Immunogen Sequence: CSNDDEDPKSHRDPSN
Antibody Immunogen Description: Corresponds to amino acid residues S124 to N138.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.4 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak reactivity with recombinant human FGFR4 on protein dot blots.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 85-105 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human FGFR1 and FGFR2.
Related Product 1: FGFR4 (124-138) antibody blocking peptide (Cat. No.: PE-01BBW99)
Related Product 2: FGFR4 expression antibody (Cat. No.: AB-NK239-1)
Related Product 3: FGFR1 expression antibody (Cat. No.: AB-NK062-3)
Related Product 4: FGFR1 Y653+Y654 phosphorylation antibody (Cat. No.: AB-PK634)
Related Product 5: FGFR2 expression antibody (Cat. No.: AB-NK063-4)
Related Product 6: FGFR2 Y656+Y657 phosphorylation antibody (Cat. No.: AB-PK635)
Related Product 7: FGFR3 expression antibody (Cat. No.: AB-NK236-3)
Related Product 8: FGFR3 Y647+Y648 phosphorylation antibody (Cat. No.: AB-PK636)
Related Product 9: FGFR3 Y647+Y648 phosphorylation antibody (Cat. No.: AB-PK637)
Scientific Background: FGFR4 is a protein-tyrosine kinase of the TK group and FGFR family. It is a receptor kinase that is involved in recognizing fibroblast growth factors to control cell migration, differentiation, proliferation, and regulating lipid metabolism. It is activated by binding of fibroblast growth factor (FGF), which induces dimerization and autophosphorylation. Interaction with PLCG1 with FGFR4 is mediated through autophosphorylation of Y754. FGFR4 may be an oncoprotein (OP). Defects in FGFR4 have been linked to alveolar rhabdomyosarcomas (ARMS), breast fibroadenomas, gliomatosis cerebri, and anaplastic astrocytomas. This description may include information annotated by UniProt and/or Google AI.