Product Name:
ErbB4-1
Product Number:
ab-nk235-1
Target Full Name: ErbB4 receptor-tyrosine kinase
Target Alias: HER4; Kinase ErbB4; P180erbB4; P180-erbB4; V-erb-a erythroblastic leukemia viral oncogene 4; MGC138404; CCDS2394.1; ENSG00000178568
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK235-1
Antibody Target Type: Pan-specific
Protein UniProt: Q15303
Protein SigNET: Q15303
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ErbB4 (HER4) sequence peptide Cat. No.: PE-01ATY85
Antibody Immunogen Sequence: LEQQYRALRKYYENC
Antibody Immunogen Description: Corresponds to amino acid residues L42 to C56; In the extracellular domain of the ErbB4 receptor.
Production Method: Corresponds to amino acid residues L42 to C56; In the extracellular domain of the ErbB4 receptor.
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with recombinant human ErbB4 on protein dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 150-170 kDa.
Antibody Specificity: Very low
Antibody Cross Reactivity: Medium-weak immunoreactivity on protein dot blots with recombinant human EGFR, and no immunoreactivity with ErbB2.
Related Product 1: ErbB4-1 blocking peptide
Related Product 2: ErbB4 pan-specific antibody (Cat. No.: AB-NK235)
Related Product 3: ErbB4-2 pan-specific antibody (Cat. No.: AB-NK235-2)
Related Product 4: ErbB4-3 pan-specific antibody (Cat. No.: AB-NK235-3)
Related Product 5: ErbB4-pY733 phosphosite-specific antibody (Cat. No.: AB-PK619)
Related Product 6: ErbB4-pY875 phosphosite-specific antibody (Cat. No.: AB-PK620)
Scientific Background: ErbB4 (HER4) is a protein-tyrosine kinase of the TK group and EGFR family. It is a receptor kinase that upon binding ligands such as neuregulins can drive signalling cascades and many cellular responses to change gene expression, cytoskeletal rearrangement, anti-apoptosis and increased cell proliferation. Phosphorylation at Y1056 induces interaction with PIK3C2A, and phosphorylation at Y188 induces interaction with Shc1. ErbB4 is a known oncoprotein (OP) and tumour suppressor protein (TSP). Downregulation of ErbB4 has been described in bladder cancer to correlated with oncogensis, but its upregulation is noted in lung cancer. Its mutation pattern in human tumours is very characteristic of a tumour suppressor protein. Mutations in ErbB4 are associated with many types of cancer. Multiple missense mutations in ErbB4 have been found in melanoma patients with increased ErbB4 phosphotransferase activity and transformation ability. Melanoma cells with mutant ErbB4 had reduced cell growth after shRNA knockdown of ErbB4 or treatment with ErbB inhibitor.
