Product Name:
TRRAP-2
Product Number:
ab-nk232-3
Target Full Name: Transformation/transcription domain-associated protein
Target Alias: 350,400 kDa PCAF-associated factor; 350/400 kDa PCAF-associated factor; PAF350,400; PAF350/400; PAF400; STAF40; Tra1; Transformation/transcription domain-associated; TRAP; TR-AP
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK232-3
Antibody Target Type: Pan-specific
Protein UniProt: Q9Y4A5
Protein SigNET: Q9Y4A5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TRRAP sequence peptide Cat. No.: PE-01ATK65
Antibody Immunogen Sequence: CETKLKMMQEVSENFE
Antibody Immunogen Description: Corresponds to amino acid residues E36 to E50
Production Method: Corresponds to amino acid residues E36 to E50
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 350-450 kDa.
Related Product 1: TRRAP-2 blocking peptide
Related Product 2: TRRAP pan-specific antibody (Cat. No.: AB-NK232)
Related Product 3: TRRAP-3 pan-specific antibody (Cat. No.: AB-NK232-1)
Scientific Background: TRRAP is a protein-serine/threonine kinase and transcription, coactivator/corepressor of the Atypical group and PIKK family. It is a multidomain kinase that belongs to the phosphoinositide-3-kinase-related kinase (PIKK) family. The TRRAP protein functions as an adaptor protein in various multiprotein chromatin complexes that collectively display histone acetyltransferase activity (HAT) and function in the epigenetic regulation of transcriptional activity. in addition, TRRAP is a component of the NuA4 histone acetyltransferase complex, which mediates the acetylation of histones H4 and H2A. TRRAP may be an oncoprotein (OP). TRRAP mediates the transcriptional activation induced by the adenovirus E1A. In addition, TRRAP plays a critical role in the regulation of Myc activation and is involved in Myc-mediated cell transformation. Several mutations (e.g. S722P in cutaneous malignant melanoma cells) in the TRRAP gene have been observed in human cancers. Knockdown of the mutant S722P TRRAP protein with siRNA in melanoma cancer cells results in increased apoptosis, indicating that the mutant TRRAP protein is essential for melanoma cell survival and tumour progression. In animal models, Cre-lox mediated knockdown of TRRAP expression lead to reduced cell proliferation due to the necessity of TRRAP for the regulation of the mitotic checkpoint and normal progression through the cell cycle.