Product Name:
MELK-1
Product Number:
ab-nk229-2
Target Full Name: Maternal embryonic leucine zipper kinase
Target Alias: hMELK; hPK38; KIAA0175; Maternal embryonic leucine zipper kinase; MELK; pEg3 kinase; Protein kinase PK38
Product Type Specific: MELK protein kinase pan-specific antibody
Antibody Code: NK229-2
Antibody Target Type: Pan-specific
Protein UniProt: Q14680
Protein SigNET: MELK
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MELK sequence peptide Cat. No.: PE-01AST85
Antibody Immunogen Sequence: MKDYDELLKYYELHEC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to E15; N-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with recombinant human MELK on protein dot blots.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Mouse | Platypus | Chicken | Frog.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 70-75 kDa.
Antibody Specificity: Very high
Related Product 1: MELK pan-specific antibody (Cat. No.: AB-NK229-1)
Related Product 2: MELK pan-specific antibody (Cat. No.: AB-NK229-2P)
Related Product 3: MELK-2 pan-specific antibody (Cat. No.: AB-NK229-3)
Related Product 4: MELK-pY438 phosphosite-specific antibody (Cat. No.: AB-PK701)
Scientific Background: MELK is an AMPK-related protein-serine/threonine kinase of the CAMK group and CAMKL family. It can occur in 8 human isoforms ranging from 74.6 to 52.5 kDa in size. It is involved in various processes such as cell cycle regulation (with high activity during the G2/M transition) and in regulation of apoptosis., self-renewal of stem cells, apoptosis and splicing regulation. MELK features an N-terminal kinase domain, a UBA domain, and a C-terminal kinase-associated 1 (KA1) domain. It is activated by autophosphorylation of the T-loop at Thr-167 and Ser-171: in contrast to other members of the SNF1 subfamily, phosphorylation at Thr-167 is not mediated by STK11/LKB1 but via autophosphorylation instead. Phosphorylation at Thr-478 induces interaction with NIPP-1. It exhibits a broad substrate specificity with targets that include BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. It is an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1, and its phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. It is required for proliferation of embryonic and postnatal multipotent neural progenitors. It phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of its pro-apoptotic function. It inhibits spliceosome assembly during mitosis by phosphorylating ZNF622, which contributes to its redirection to the nucleus. It interacts with ZNF622 and PPP1R8. It is expressed in placenta, kidney, thymus, testis, ovary and intestine. It is highly expressed in various cancers—especially basal-like breast cancer—and in immature neural stem cells. This description may include information annotated by UniProt and/or Google AI.