Product Name:
VEGFR1-1
Product Number:
ab-nk226-2
Target Full Name: Vascular endothelial growth factor receptor 1
Target Alias: FLT; Flt-1; Fms-like tyrosine kinase 1; Fms-related tyrosine kinase 1; FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; VGFR1; CCDS9330.1; ENSG00000102755
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK226-2
Antibody Target Type: Pan-specific
Protein UniProt: P17948
Protein SigNET: P17948
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human VEGFR1 (Flt1) sequence peptide Cat. No.: PE-01ASD85
Antibody Immunogen Sequence: PEIIHMTEGRELVIPC
Antibody Immunogen Description: Corresponds to amino acid residues P143 to C158; Just before and part of the second Ig-like C2-type domain
Production Method: Corresponds to amino acid residues P143 to C158; Just before and part of the second Ig-like C2-type domain
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with recombinant human VEGFR1 on protein dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 150-190 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human VGFR2 and VGFR3.
Related Product 1: VEGFR1-1 blocking peptide
Related Product 2: VEGFR1 pan-specific antibody (Cat. No.: AB-NK226-2P)
Related Product 3: VEGFR1-pY1048 phosphosite-specific antibody (Cat. No.: AB-PK850)
Related Product 4: VEGFR1-pY1053 phosphosite-specific antibody (Cat. No.: AB-PK851)
Related Product 5: VEGFR1-1 pan-specific antibody (Cat. No.: AB-NK226-2)
Scientific Background: VEGFR1 (Flt1) is a protein-tyrosine kinase of the TK group and VEGFR family. It functions as a membrane receptor for VEGFA, VEGFB, and PGF. Binding vascular endothelial growth factor (VEGF), which induces dimerization and autophosphorylation. Autophosphorylation of Y1169 induces interaction with PLCg1. Autophosphorylation of Y1213 increases phosphotransferase activity and induces interaction with Grb2, PLCg1 and SHP2. Autophosphorylation ofY1333 induces interaction with Cbl, Crk, Nck1 and PLCg1. VEGFR1 is critical for the development of embryonic vasculature, angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. In adulthood, VEGFR1 promotes endothelial cell proliferation, survival, and angiogenesis. In addition, the VEGFR1 protein mediates the activation of the MAPK and Akt1 intracellular signalling pathways, as well as directly phosphorylates Src, Yes1, and possibly Cbl. VEGFR1 appears to be an oncoprotein (OP). Cancer-related mutations in human tumours point to a gain of function of the protein kinase. Gain-of-function mutations in the VEGFR1 gene are associated with cancer development. VEGFR1 expression has been shown to contribute to cancer cell survival, proliferation, migration, tissue invasion, tumour angiogenesis, and metastasis. In addition, VEGFR1 may promote cancer pathogenesis by enhancing inflammatory responses and recruiting tumour-infiltrating macrophages.
