Product Name:
MEKK6-2
Product Number:
ab-nk225-2
Target Full Name: Mitogen-activated protein kinase kinase kinase 6
Target Alias: Apoptosis signal regulating kinase 2; Kinase MAP3K6; M3K6; MAP3K6; MAPKKK6; MEKK6; MEK kinase 6; MGC125653; MGC20114; MGC125653; CCDS299.1; ENSG00000142733
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK225-2
Antibody Target Type: Pan-specific
Protein UniProt: O95382
Protein SigNET: O95382
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MEKK6 (MAP3K6) sequence peptide Cat. No.: PE-01ASR95
Antibody Immunogen Sequence: CGKRSRSPSSPRHAPR
Antibody Immunogen Description: Corresponds to amino acid residues G909 to R923; Post-kinase last alpha-chain
Production Method: Corresponds to amino acid residues G909 to R923; Post-kinase last alpha-chain
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 110-130 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human MEKK2 and YSK4.
Related Product 1: MEKK6-2 blocking peptide
Related Product 2: MEKK6 pan-specific antibody (Cat. No.: AB-NK225-1)
Related Product 3: MEKK6 pan-specific antibody (Cat. No.: AB-NK225-2P)
Related Product 4: MEKK6 pan-specific antibody (Cat. No.: AB-NK225-3P)
Related Product 5: MEKK6-1 pan-specific antibody (Cat. No.: AB-NK225-4)
Related Product 6: MEKK6-3 pan-specific antibody (Cat. No.: AB-NK225-3)
Scientific Background: MEKK6 (ASK2, MAP3K6) is a protein-serine/threonine kinase of the STE group and STE11 family. It apparently regulates the JNK, but not ERK or p38 kinase pathways. It is activated by phosphorylation on T806 in the kinase activation loop. It is catalytically active only when complexed with MAP3K5, with MAP3K5 supporting the stability and the active configuration of MAP3K6, and MAP3K6 activating MAP3K5 by direct phosphorylation. It may be a tumour suppressor protein (TSP). However, its extremely low rate of mutation in human tumours indicates that it may also act as a tumour requiring protein (TRP). MEKK6 can form a heterocomplex with MAP3K5 (ASK1) to act as a proapoptotic signal and tumour suppressor protein. The P946L, F849Sfs*142, P958T, D200Y and V207G variants have been linked to familial gastric cancer, and they were predicted to be variants that lead to either a decrease in function or dominant negative phenotype. Studies with mouse models also support the role of ASK2 as a tumour suppressor.
