Product Name:
S6K-III
Product Number:
ab-nk223-4
Target Full Name: Ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase 1
Target Alias: RPS6KB1; KS6B1; Ps6K; p70(S6K)-alpha; Ribosomal protein S6 kinase I; RPS6KB1; S6K; S6K1; STK14A
Product Type Specific: RPS6KB1 (p70S6Kb) protein kinase pan-specific antibody
Antibody Code: NK223-4
Antibody Target Type: Pan-specific
Protein UniProt: P23443
Protein SigNET: S6K
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human p70S6K (S6Ka, RPS6KB1) sequence peptide Cat. No.: PE-01AQP75
Antibody Immunogen Sequence: AMIVRNAKDTAHTKAERNILEEVKHP-GGC
Antibody Immunogen Description: Corresponds to amino acid residues A128 to P153; Kinase catalytic subdomain 3.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in A431 and HEK-293 cells.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 63-70 kDa.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: A very strong 26-28 kDa cross-reactive protein was detected in many mouse tissues and diverse human cancer cell lines. Strong 100 kDa cross-reactive protein was also detected in human A431, HEK-293 and Jurkat cells. A strong 50 kDa cross-reactive protein in mouse brain and human Jurkat cells, which may be a S6K isoform.
Related Product 1: S6K-III blocking peptide
Related Product 2: S6KASubtide - p70S6K (RPS6KB1) protein kinase substrate peptide
Related Product 3: S6KBSubtide kinase substrate peptide (Cat. No.: PE-01BJI85)
Related Product 4: RPS6 (230-238) KinSub kinase substrate peptide (Cat. No.: PE-01ADQ95)
Related Product 5: S6K expression antibody (Cat. No.: AB-NK223-2)
Related Product 6: RSK1 expression antibody (Cat. No.: AB-NK164-3)
Related Product 7: RSK1 S221 phosphorylation antibody (Cat. No.: AB-PK804)
Related Product 8: RSK1 S380 phosphorylation antibody (Cat. No.: AB-PK805)
Related Product 9: RSK1 T573 phosphorylation antibody (Cat. No.: AB-PK806)
Related Product 10: RSK2 expression antibody (Cat. No.: AB-NK165-3)
Scientific Background: p70S6K (RPS6KB1) is a protein-serine/threonine kinase that is a member of the AGC group of protein kinases in the RSK family, and p70 subfamily. This kinase is highly expressed and widely distributed in most tested human tissues except in the brain and spinal cord where it is more moderately expressed. It functions downstream of mTOR (FRAP1) signalling in response to growth factors to promote cell proliferation, growth, and progression through the cell cycle. p70S6K is activated by phosphorylation at Thr-252 (by PDK1), Ser-394 (by mTOR), Thr-412 (by mTOR), Ser-427, Ser-434 (by ERK1/2), Ser-441, Thr-444 (by ERK1/2) and Ser-447 (by ERK1/2). It plays a crucial role in promoting protein synthesis, cell growth, and proliferation. Acting downstream of the mTORC1 signaling pathway, it phosphorylates the S6 ribosomal protein, which stimulates ribosome function and translation, particularly in response to growth factors and nutrients. It also targets components such as EIF4B, and EEF2K to boost protein synthesis. Its activation by serum stimulation is inhibited by wortmannin and rapamycin. p70S6K phosphotransferase activity changes during the cell cycle, and increases 20-fold in G1 cells released from G0. It has been linked with the development of diet-induced obesity, colon adenocarcinoma and breast cancer. It is often overexpressed in cancer (e.g., breast cancer) and linked to metabolic diseases like obesity and diabetes. This description may include information annotated by UniProt and/or Google AI.