Product Name:
JNK1-2
Product Number:
ab-nk217-3
Target Full Name: Jun N-terminus protein-serine kinase (stress-activated protein kinase (SAPK)) 1
Target Alias: c-Jun N-terminal kinase 1; JNK; JNK1A2; JNK1-alpha-2; JNK-46;JNK21B1/2; JUN N-terminal kinase; Kinase JNK1; MAPK8; MK08; PRKM8; SAPK1; SAPK1c; Stress-activated protein kinase JNK1; CCDS7225.1; ENSG00000107643
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK217-3
Antibody Target Type: Pan-specific
Protein UniProt: P45983
Protein SigNET: P45983
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human JNK1 (MAPK8) sequence peptide Cat. No.: PE-01AXL99
Antibody Immunogen Sequence: CLGAAVINGSQHPSSS
Antibody Immunogen Description: Corresponds to amino acid residues L379 to S393
Production Method: Corresponds to amino acid residues L379 to S393
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 43-46 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human JNK2 and JNK3.
Related Product 1: JNK1-2 blocking peptide
Related Product 2: JNK1-1 pan-specific antibody (Cat. No.: AB-NK217-2)
Related Product 3: JNK1-pY185 phosphosite-specific antibody (Cat. No.: AB-PK670)
Related Product 4: JNK2-1 pan-specific antibody (Cat. No.: AB-NK189-2)
Related Product 5: JNK2-3 pan-specific antibody (Cat. No.: AB-NK189-4)
Related Product 6: SAPKa/b (JNK2-4) pan-specific antibody (Cat. No.: AB-NK196)
Related Product 7: JNK3-1 pan-specific antibody (Cat. No.: AB-NK197-2)
Related Product 8: JNK3-3 pan-specific antibody (Cat. No.: AB-NK197-4)
Related Product 9: JNKSelectideA - JNK1 protein kinase substrate peptide
Related Product 10: JNKSubtide - JNK1 (MAPK8) protein kinase substrate peptide
Scientific Background: JNK1 (MAPK8) is a protein-serine/threonine kinase of the CMGC group and MAPK family. It is involved in the regulation of several cellular processes, including cell proliferation, differentiation, migration, transformation, and apoptosis, through the JNK/SAP signalling pathway. This pathway is activated by extracellular stimuli such as pro-inflammatory cytokines or physical stress, as well as signals of intracellular stress, such as misfolded proteins in the endoplasmic reticulum. Phosphorylation of T183 and Y185 by either of two dual specificity kinases, MAP2K4 and MAP2K7, increases phosphotransferase activity. It is inhibited by dual specificity phosphatases, such as DUSP1, which target the T183 and Y185 activatory phosphorylation sites.
