Product Name:
ERK5-NT (ERK5-4)
Product Number:
ab-nk206-2
Target Full Name: Extracellular regulated protein-serine kinase 5 (Big MAP kinase 1 (BMK1))
Target Alias: BMK; BMK1; BMK1 kinase; ERK4; ERK-5; Extracellular-signal-regulated kinase 5; Kinase ERK5; MAPK7; PRKM7; ENSG00000166484
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK206-2
Antibody Target Type: Pan-specific
Protein UniProt: Q13164
Protein SigNET: Q13164
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ERK5 (MAPK7) sequence peptide
Antibody Immunogen Sequence: MAEPLKEEDGEDGSC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to S14; N-terminus
Production Method: Corresponds to amino acid residues M1 to S14; N-terminus
Antibody Modification: Protein kinase pan-specific antibody
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Mouse; Rat
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 88-115 kDa.
Antibody Cross Reactivity: Cross-reacts with unidentified 49 and 28 kDa proteins in various rat tissues.
Related Product 1: ERK5-2 pan-specific antibody (Cat. No.: AB-NK206-4)
Related Product 2: ERK5-3 pan-specific antibody (Cat. No.: AB-NK206-5)
Related Product 3: ERK5-pT219+pY221 phosphosite-specific antibody (Cat. No.: AB-PK625)
Related Product 4: ERK5-pY221 phosphosite-specific antibody (Cat. No.: AB-PK626)
Related Product 5: ERK5 -PNT (ERK5-5) pan-specific antibody (Cat. No.: AB-NK206-1)
Scientific Background: ERK5 (MAPK7) is a protein-serine/threonine kinase of the CMGC group and MAPK family. It is important in controlling a cell's proliferation, differentiation, and cell survival. Phosphorylation of T219 and Y221 by MEK5 (MAP2K5) increases the phosphotransferase activity of ERK5. MEK5 is activated in response to hyperosmolarity, hydrogen peroxide, and epidermal growth factor (EGF). ERK5 may be a tumour requiring protein (TRP). Although there has been no direct linkage of ERK5 to formation of carcinomas in humans in vivo, in vitro ERK5 over-expression has been shown to increase metastasis, and ERK5 inhibition has been shown to inhibit osteosarcoma cell motility. Upregulation of the MEK5 gene has been noted in breast, prostate, and liver cancer.