Product Name:
PKG1b-NT (PKG1b-1)
Product Number:
ab-nk203
Target Full Name: cGMP-dependent protein kinase 1, beta isozyme
Target Alias: cGK 1 alpha; cGKI-alpha; cGKI-beta; cGMP-dependent protein kinase 1, alpha isozyme; DKFZp686K042; FLJ36117; KGP1A; Kinase PKG1; PGK; PKG; PKG1-alpha; Protein kinase, cGMP-dependent, type I
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK203
Antibody Target Type: Pan-specific
Protein UniProt: Q13976-2
Protein SigNET: Q13976-2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PKG1b (PRKG1B) sequence peptide
Antibody Immunogen Sequence: CLRDLQYALQEKIEE
Antibody Immunogen Description: Corresponds to amino acid residues L4 to E17; N-terminus
Production Method: Corresponds to amino acid residues L4 to E17; N-terminus
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Mouse; Rat
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 77-83 kDa.
Related Product 1: PKG1-BKCD pan-specific antibody (Cat. No.: AB-NK202-2)
Related Product 2: PKG1-pT515+pT517 phosphosite-specific antibody (Cat. No.: AB-PK776)
Related Product 3: PKG1a-NT (PKG1a-1) pan-specific antibody (Cat. No.: AB-NK202)
Related Product 4: PKG1Subtide - PKG1 (PRKG1) protein kinase substrate peptide
Scientific Background: PKG1 (PRKG1) is a protein-serine/threonine kinase of the AGC group and PKG family. It is activated by cGMP, which is generated in response to nitric oxide. Phosphorylation at T59 increases phosphotransferase activity and its affinity for cGMP Phosphorylation at T517 increases phosphotransferase activity. An R177Q mutation in AAT8 results in a constitutively active form of PKG1, which also has reduced cGMP binding.