Product Name:
PKG1a-NT (PKG1a-1)
Product Number:
ab-nk202
Target Full Name: cGMP-dependent protein kinase 1, alpha isozyme
Target Alias: cGK 1 alpha; cGKI-alpha; cGKI-beta; cGMP-dependent protein kinase 1, alpha isozyme; DKFZp686K042; FLJ36117; KGP1A; Kinase PKG1; PGK; PKG; PKG1-alpha; Protein kinase, cGMP-dependent, type I
Product Type Specific: PKG1 (PRKG1) protein kinase pan-specific antibody
Antibody Code: NK202
Antibody Target Type: Pan-specific
Protein UniProt: Q13976
Protein SigNET: PKG1a
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PKG1a (PRKG1A) sequence peptide
Antibody Immunogen Sequence: ELEEDFAKILMLKEEC
Antibody Immunogen Description: Corresponds to amino acid residues E3 to E17; N-terminus.
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Dog | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 77-83 kDa.
Related Product 1: PKG1Subtide kinase substrate peptide (Cat. No.: PE-01BIW95)
Related Product 2: PKG1 expression antibody (Cat. No.: AB-NK202-2)
Related Product 3: PKG1 T515+T517 phosphorylation antibody (Cat. No.: AB-PK776)
Related Product 4: PKG2 expression antibody (Cat. No.: AB-NK290-1)
Related Product 5: PKG2 expression antibody (Cat. No.: AB-NK290-2)
Scientific Background: PKG1 (PRKG1) is a protein-serine/threonine kinase of the AGC group and PKG family. It is activated by cGMP, which is generated in response to nitric oxide. As a key downstream effector of the nitric oxide (NO)/cGMP signalling pathway, it primarily regulates cardiovascular homeostasis and smooth muscle relaxation. It exists as a homodimer and regulates intracellular calcium levels to inhibit platelet activation, lower blood pressure, and protect against cardiac hypertrophy and fibrosis. Two main isoforms, PKG1α and PKG1β, are generated from the PRKG1 gene, differing in their N-terminal dimerization domains. PKG1α features a leucine/isoleucine zipper (LZ) domain that mediates homodimerization and provides high affinity for cGMP, acting as a crucial regulator in the cardiovascular system. PKG1β contains a distinct N-terminal domain, which can modulate its own activity. Phosphorylation at Thr-59 increases phosphotransferase activity and its affinity for cGMP Phosphorylation at Thr-517 increases phosphotransferase activity. An R177Q mutation in AAT8 results in a constitutively active form of PKG1, which also has reduced cGMP binding. It is highly expressed in smooth muscle, heart, lungs, and the central nervous system (CNS). PKG1 is associated with familial thoracic aortic aneurysm and dissection (AAT8), and is a key target for treating hypertension and cardiac diseases. This description may include information annotated by UniProt and/or Google AI.