Product Name:
KKIALRE-CT (CDKL1-1)
Product Number:
ab-nk199
Target Full Name: Cyclin-dependent kinase-like 1; Serine/threonine protein kinase KKIALRE
Target Alias: Cyclin-dependent kinase-like 1; CDKL1
Product Type Specific: CDKL1 (KKIALRE) protein kinase pan-specific antibody
Antibody Code: NK199
Antibody Target Type: Pan-specific
Protein UniProt: Q00532
Protein SigNET: CDKL1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDKL1 sequence peptide
Antibody Immunogen Sequence: CLDNKKYYSDTKKLNYR
Antibody Immunogen Description: Corresponds to amino acid residues L338 to R353; C-terminus.
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Platypus.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-44 kDa.
Related Product 1: CDKL2 expression antibody (Cat. No.: AB-NK261-1)
Related Product 2: CDKL3 expression antibody (Cat. No.: AB-NK262-1)
Related Product 3: CDKL5 Y171 phosphorylation antibody (Cat. No.: AB-PK576)
Scientific Background: CDKL1 (KKIALRE) is a protein-serine/threonine kinase of the CMGC group and CDKL family, and functions in cell cycle progression, proliferation, and cilia length regulation. The deduced CDKL1 protein is 358-amino acids long and has 43% sequence identity to CDC2. However, it was identified as a human homologue of cyclin-dependent kinases by low stringency cloning studies. In view of its size and the conservation of a Thr-X-Tyr dual phosphorylation site motif in the region between kinase subdomains VII and VIII, it is more likely that KKAILRE corresponds to a MAP kinase family member. CDKL1 is highly expressed in tissues such as the brain, lungs, kidneys, and ovaries. CDKL1 may be an oncoprotein (OP). Significantly elevated CDKL1 expression was observed in gastric cancer tissue specimens. Overexpression of CDKL1 promotes tumour cell proliferation and survival, while inhibiting it induces cell cycle arrest (G0/G1 or G2/M phases) and apoptosis. In addition, loss of CDKL1 function in gastric cancer cell lines (SGC7901 and MGC-803) resulted in impaired cell proliferation and enhanced apoptosis. Furthermore, siRNA-mediated knockdown of CDKL1 expression stimulated the pro-apoptotic factor Bcl-2-interacting killer (BIK) and reduced cellular expression of the proliferating cell nuclear antigen (PCNA), indicating reduced cellular proliferation. It is commonly overexpressed in cancers like breast and gastric cancer, where it supports tumor survival, and is largely localized in the nucleus. This description may include information annotated by UniProt and/or Google AI.