Product Name:
SAPKb-NT (JNK3-4)
Product Number:
ab-nk197
Target Full Name: Jun N-terminus protein-serine kinase (stress-activated protein kinase (SAPKb)) 3
Target Alias: c-Jun N-terminal kinase 3; FLJ12099; FLJ33785; JNK3A; JNK3-alpha-2; Kinase JNK3; MAP kinase p49 3F12; MAPK10; MGC50974; MK10; P493F12; p54bSAPK; PRKM10; Stress-activated protein kinase JNK3; PRKM10; CCDS3613.1; ENSG00000109339
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK197
Antibody Target Type: Pan-specific
Protein UniProt: P53779
Protein SigNET: P53779
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human JNK3 (MAPK10) sequence peptide
Antibody Immunogen Sequence: MSDSKSDGQFYSVQVAC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to A16; N-terminus
Production Method: Corresponds to amino acid residues M1 to A16; N-terminus
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Western blots with recombinant rat JNK3 expressed in E. coli.
Antibody Species Reactivity: Human; Mouse; Rat
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 48-54 kDa.
Antibody Cross Reactivity: No immunoreactivity with recombinant rat JNK2 expressed in E. coli.
Related Product 1: JNK1-1 pan-specific antibody (Cat. No.: AB-NK217-2)
Related Product 2: JNK1-2 pan-specific antibody (Cat. No.: AB-NK217-3)
Related Product 3: JNK1-pY185 phosphosite-specific antibody (Cat. No.: AB-PK670)
Related Product 4: JNK2-1 pan-specific antibody (Cat. No.: AB-NK189-2)
Related Product 5: JNK2-2 pan-specific antibody (Cat. No.: AB-NK189-3)
Related Product 6: SAPKa/b (JNK2-4) pan-specific antibody (Cat. No.: AB-NK196)
Related Product 7: JNK3-1 pan-specific antibody (Cat. No.: AB-NK197-2)
Related Product 8: JNK3-2 pan-specific antibody (Cat. No.: AB-NK197-3)
Related Product 9: JNKSelectideA - JNK1 protein kinase substrate peptide
Related Product 10: JNKSubtide - JNK1 (MAPK8) protein kinase substrate peptide
Scientific Background: JNK3 (MAPK10) is a protein-serine/threonine kinase of the CMGC group and MAPK family. Phosphorylation at T221 and Y223, by MAP2K4 and MAP2K7. MAP2K7, increases phosphotransferase activity. MAP2K7 phosphorylates JNK3 on T221 causing a conformational change and a large increase in Vmax. MAP2K4 then phosphorylates Y223 resulting in a further increase in Vmax. Phosphorylation at T131 inhibits phosphotransferase activity. It is inhibited by dual specificity phosphatases, such as DUSP1 and by HDAC9. The near absence of reported somatic mutations in this protein indicates that it may function as a tumour requiring protein (TRP) in many human tumours.
