Product Name:
JNK2-4 (SAPKa/b)
Product Number:
ab-nk197
Target Full Name: Jun N-terminus protein-serine kinase (stress-activated protein kinases 1 and 3
Target Alias: c-Jun N-terminal kinase 2; JNK2alpha; JNK2-alpha-2; JNK2A; JNK2B; JNK2beta; JNK-55; Jun kinase; Kinase JNK2; MAPK9; MK09; p54a; PRKM9; Stress-activated protein kinase JNK2; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; CCDS4453.1; ENSG00000050748
Product Type Specific: JNK2 (MAPK9) protein kinase pan-specific antibody
Antibody Code: NK197
Antibody Target Type: Pan-specific
Protein UniProt: P45983
Protein SigNET: JNK2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Rat JNK2 (MAPK9) sequence peptide
Antibody Immunogen Sequence: MSDSKSDGQFYSVQVAC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to A16 of rat JNK2; N-terminus.
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Western blots with recombinant rat JNK2 expressed in E. coli.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Platypus | Frog.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 48-54 kDa.
Antibody Cross Reactivity: No immunoreactivity with recombinant rat JNK2 expressed in E. coli.
Related Product 1: JNKSelectideA kinase substrate peptide (Cat. No.: PE-14CAD90)
Related Product 2: JNKSubtide kinase substrate peptide (Cat. No.: PE-01BHT95)
Related Product 3: JNK2 T183+Y185 phosphorylation antibody (Cat. No.: AB-PK902)
Related Product 4: JNK2 expression antibody (Cat. No.: AB-NK189-2)
Related Product 5: JNK2 expression antibody (Cat. No.: AB-NK189-3)
Related Product 6: JNK2 expression antibody (Cat. No.: AB-NK189-4)
Related Product 7: JNK1 expression antibody (Cat. No.: AB-NK217-3)
Related Product 8: JNK3 expression antibody (Cat. No.: AB-NK197)
Related Product 9: JNK3 expression antibody (Cat. No.: AB-NK197-3)
Related Product 10: JNK3 expression antibody (Cat. No.: AB-NK197-4)
Scientific Background: JNK2 (MAPK9) is a protein-serine/threonine kinase of the CMGC group and MAPK family. JNK2 is expressed as several isoforms (46 kDa and 54 kDa) through alternative splicing, mainly differing in their C-terminus. It is involved in the control of cell differentiation, transformation, migration, proliferation, and programmed cell death in response to stress and cytokines. JNK2 is known to play a critical role in T-cell differentiation and apoptosis, by preventing ubiquitination of p53 in non-stress cells. Phosphorylation of Thr-183 and Tyr-185 by either of two dual specificity kinases, MAP2K4 and MAP2K7, increases phosphotransferase activity. Phosphorylation of Ser-129 also increases phosphotransferase activity. It is inhibited by dual specificity phosphatases, such as DUSP1, which target the Thr-183 and Tyr-185 activatory phosphorylation sites. JNK2 often functions as a positive regulator of c-Jun, though it can also act as a negative regulator of cell proliferation, unlike JNK1. JNK2 has been linked to several pathologies, including cancer (e.g., lung carcinoma transformation) and inflammation. Studies also indicate its role in promoting cell survival during endoplasmic reticulum (ER) stress. JNK2 has been shown, in a cancer cell line to have a role in breast cancer cell motility. This description may include information annotated by UniProt and/or Google AI.