Product Name:
SGK3-2
Product Number:
ab-nk170-4
Target Full Name: Serine-threonine-protein kinase Sgk3
Target Alias: CISK; EC 2.7.11.1; Serine/threonine protein kinase CISK; Serum/glucocorticoid regulated kinase family, member 3; Serum/glucocorticoid-regulated kinase 3; Serum/glucocorticoid-regulated kinase-like; SGK2; SGKL; SGK-like protein SGKL
Product Type Specific: SGK3 protein kinase pan-specific antibody
Antibody Code: NK170-4
Antibody Target Type: Pan-specific
Protein UniProt: Q96BR1
Protein SigNET: SGK3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human SGK3
Antibody Immunogen Sequence: CGVSLTAWSILEELLEKDRQ
Antibody Immunogen Description: Corresponds to amino acid residues G383 to A406.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus.
Related Product 1: SGK1 expression antibody (Cat. No.: AB-NK294-1)
Related Product 2: SGK3 expression antibody (Cat. No.: AB-NK170-3)
Scientific Background: SGK3 is protein-serine kinase in ther AGC group and SGK family. Two specific sites, one in the kinase domain (Thr-320) and the other in the C-terminal regulatory region (Ser-486), need to be phosphorylated for its full activation. It is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. It up-regulates Na+ channels: SCNN1A/ENAC and SCN5A, K+ channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca2+ channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na+/dicarboxylate cotransporter: SLC13A2/NADC1, Na+-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na+/H+ exchanger: SLC9A3/NHE3, and the Na+/K+ ATPase. Substrates include NDEDD4L, GSK3B, FLII, ITCH/AIP4. SGK3 can be induced by androgen, and it may promote prostate cancer cell proliferation. This description may include information annotated by UniProt and/or Google AI.