Product Name:
Ron-1
Product Number:
ab-nk161-2
Target Full Name: Macrophage-stimulating protein receptor alpha chain
Target Alias: CD136; CD136 antigen; CDW136; C-met-related tyrosine kinase; Kinase RON; Macrophage stimulating 1 receptor; Macrophage-stimulating protein receptor precursor; MSP receptor; MST1R; PTK8; STK; CCDS2807.1; ENSG00000164078
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK161-2
Antibody Target Type: Pan-specific
Protein UniProt: Q04912
Protein SigNET: Q04912
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Ron (RONa) sequence peptide Cat. No.: PE-01AVD95
Antibody Immunogen Sequence: CLRDLDSALLAEVKDV
Antibody Immunogen Description: Corresponds to amino acid residues R1060 to V1073
Production Method: Corresponds to amino acid residues R1060 to V1073
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium-strong immunoreactivity with recombinant human Ron on protein dot blots.
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 150-220 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human Met.
Related Product 1: Ron-1 blocking peptide
Related Product 2: Ron-2 pan-specific antibody (Cat. No.: AB-NK161-3)
Related Product 3: Ron-3 pan-specific antibody (Cat. No.: AB-NK161-4)
Related Product 4: Ron-pY1238 phosphosite-specific antibody (Cat. No.: AB-PK800)
Related Product 5: Ron-pY1238 +pY1239 phosphosite-specific antibody (Cat. No.: AB-PK801)
Related Product 6: RonSubtide - RON protein kinase substrate peptide
Related Product 7: IRS1 (979-989) KinSub - Insulin receptor substrate 1 (K979-G989, mouse) peptide; Insulin receptor substrate
Scientific Background: Ron (MST1R) is a protein-tyrosine kinase of the TK group and Met family. It is a receptor kinase that binds macrophage stimulating factor 1 (MST1, MSF) to propagate a cell migration, survival, or differentiation signal. This kinase is highly expressed and widely distributed in most tested human tissues. Phosphorylation of Ron at Y1198 and Y1288 increases phosphotransferase activity. Phosphorylation at S1394 induces interaction with 14-3-3 beta. Ron interacts with the HYAL2 receptor protein, rendering it functionally inactive. HYAL2 is a candidate tumour-suppressor glycosylphosphatidylinositol-anchored cell-surface protein that serves as an entry receptor for jaagsiekte retrovirus, a virus that causes contagious lung cancer in sheep that is morphologically similar to human bronchioloalveolar carcinoma. It was shown that Ron liberated from the association with HYAL2 becomes functionally active and activates the Akt and mitogen-activated protein kinase pathways. Ron has also been linked with the development of breast, head and neck, ovarian, renal and breast cancers, and pulmonary adenomas.
