Product Name:
PKCb (PKCb-1)
Product Number:
ab-nk133-2
Target Full Name: Protein-serine kinase C beta 1
Target Alias: Kinase PKC-beta; KPCB; MGC41878; PKC II; PKC-B; PKC-beta; PKC-II; PRKCB; PRKCB1; PRKCB2; Protein kinase C, beta type
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK133-2
Antibody Target Type: Pan-specific
Protein UniProt: P05771
Protein SigNET: P05771
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PKCb (PRKCB1) sequence peptide Cat. No.:
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 2.5 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Mouse; Rat
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 75-85 kDa.
Related Product 1: PKCb-pT500 phosphosite-specific antibody (Cat. No.: AB-PK766)
Related Product 2: PKCb-pS661 phosphosite-specific antibody (Cat. No.: AB-PK765)
Related Product 3: CREB1 (123-135) KinSub - cyclic-AMP response element binding protein-1 (K123-R135, human) peptide; Crebtide protein kinase substrate peptide
Related Product 4: CREB1 (123-135) KinSub, biotinyl. - cyclic-AMP response element binding protein-1 (K123-R135, human) peptide; Crebtide protein kinase substrate peptide
Related Product 5: PKCA/BSubtide - PKCa (PRKCA) protein kinase substrate peptide
Related Product 6: PKCtide KinSub - Protein kinase C peptide substrate
Related Product 7: Syntide-2 KinSub - Syntide-2 protein kinase substrate peptide
Scientific Background: PKCb (PRKCB, PKC-beta) is a protein-serine/threonine kinase in the classical protein kinase C family. It is calcium activated, and dependent on acidic phospholipids (e.g. phosphatidylserine) and diacylglycerol for full phosphotransferase activity. PKCb can bind 3 calcium ions for each subunit. Phosphorylation of T635 and T642 is critical for kinase activity. Phosphorylation of S661 inhibits membrane binding and causes release into cytoplasm. PKCbI is highly related to PKCbII. Unlike the mature PKCb II mRNA and protein, which rapidly increase following acute insulin treatment, the PKCbI mRNA and protein levels remain unchanged. Stable overexpression of PKCbII, but not PKCbI, leads to insulin-stimulated glucose uptake into cells. PKCb can mediate B-cell receptor signalling, and apoptosis induced by oxidative stress. PKCb can regulate oxidative stress inducing apoptosis, signalosome, B-cell activation, transcription regulation dependent on androgen receptor signalling, endothelial cell proliferation, and insulin secretion. PKCb can phosphorylate CARD11/CARMA1 at S559, S644, and S652. Upon stimulation of B cells and mast cells, Syk regulates Btk, and Btk selectively regulates enzymatic activity of PKCbI. Specific regulation of PKCbI by Btk is consistent with the selective association of Btk with PKCbI. PKCb has been linked with the development of diabetic nephropathy, colorectal adenocarcinomas and glioblastoma multiforme. PKCb may be an oncoprotein (OP), although it may also be a tumour suppressor protein, since it is actually down-regulated by phorbol esters and other tumour promoters. A higher Gleason Score significantly associated with increased PKCb expression in prostate cancer.