Product Name:
Akt2 (PKB beta)
Product Number:
ab-nk130-3
Target Full Name: RAC-beta serine/threonine-protein kinase
Target Alias: AKT2; PKB; PKB beta; PRKBB; protein kinase Akt2; RAC BETA
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK130-3
Antibody Target Type: Pan-specific
Protein UniProt: P31751
Protein SigNET: P31751
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: A five residue synthetic peptide based on the human Akt2, coupled to KLH
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 0.25 mg/ml
Storage Buffer: TBS, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting, ELISA
Antibody Dilution Recommended: 1:3000 dilution was sufficient for detection of PKBβ in 20μg of HeLa cell lysate by ECL immunoblot analysis.
Antibody Potency: Very high potency. Detects a 65 kDa protein, corresponding to the molecular mass of Akt2 PKBβ on SDS-PAGE immunoblots.
Antibody Species Reactivity: Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Pig, Rabbit, Sheep, Xenopus.
Antibody Positive Control: A 1:3000 dilution of SPC-148 was sufficient for detection of PKBβ in 20 μg of HeLa cell lysate by ECL immunoblot analysis.
Antibody Specificity: Seems very high
Antibody Cross Reactivity: Does not react with PKBα (Akt1) or PKBγ (Akt3).
Related Product 1: Akt2 pan-specific antibody (Cat. No.: AB-NK130-9P)
Related Product 2: Akt2-1 pan-specific antibody (Cat. No.: AB-NK130-4)
Related Product 3: Akt2-2 pan-specific antibody (Cat. No.: AB-NK130-8)
Related Product 4: Akt2-3 pan-specific antibody (Cat. No.: AB-NK130-9)
Related Product 5: PKB2-PCT (Akt2-4) pan-specific antibody (Cat. No.: AB-NK130-5)
Related Product 6: AktSubtide - Akt1 (PKBa) protein kinase substrate peptide
Related Product 7: GSK3b (3-12) KinSub - Glycogen synthase kinase 3-beta (GSK3b) N-terminus (G3-E12, human) peptide; Akt protein kinase substrate peptide
Scientific Background: Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).