Product Name:
Akt2 (PKB beta)
Product Number:
ab-nk130-3
Target Full Name: RAC-beta serine/threonine-protein kinase
Target Alias: Akt2; PKB beta; RAC-beta serine,threonine protein kinase; RAC-PK-beta; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA; CCDS12552.1; ENSG00000105221
Product Type Specific: Akt2 (PKBb) protein kinase pan-specific antibody
Antibody Code: NK130-3
Antibody Target Type: Pan-specific
Protein UniProt: P31751
Protein SigNET: Akt2 (PKB beta)
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: A five residue Synthetic peptide patterned after the human Akt2
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: TBS, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting, ELISA
Antibody Dilution Recommended: 1:3000 dilution was sufficient for detection of PKBβ in 20μg of HeLa cell lysate by ECL immunoblot analysis.
Antibody Potency: Very high potency. Detects a 65 kDa protein, corresponding to the molecular mass of Akt2 PKBβ on SDS-PAGE immunoblots.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Monkey | Cow | Pig | Sheep | Dog | Rabbit | Guinea pig | Rat | Mouse | Hamster | Chicken | Frog.
Antibody Positive Control: A 1:3000 dilution of SPC-148 was sufficient for detection of PKBβ in 20 μg of HeLa cell lysate by ECL immunoblot analysis.
Antibody Specificity: Seems very high
Antibody Cross Reactivity: Does not react with PKBα (Akt1) or PKBγ (Akt3).
Related Product 1: Akt2 expression antibody (Cat. No.: AB-NK130-4)
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Scientific Background: Akt2 (PKBB, PRKBB) is a protein-serine/threonine kinase of the AGC group and Akt family. Its cell signalling activities involve activating cell surival and proliferation, increasing metabolism, and angiogenesis. Its interaction network contains over 100 substrates which includes inhibiting p53 via downstream signalling, activating mTOR, inhibition of GSK3b, and inhibition of cyclins. Phosphorylation of T309 and S474 increases phosphotransferase activity. Cancer-related mutations in human tumours point to a gain of function of the protein kinase. Insertional mutagenesis studies in mice also support a role for this protein kinase in mouse cancer oncogenesis. The active form of the protein kinase normally acts to promote tumour cell proliferation. In many cancers, Akt2 is found to be overexpressed and amplified. It is also linked with invasion, cell migration and induction of angiogenesis. It is important in cell survival regulation, insulin signalling, angiogenesis and tumour formation. Defects in Akt2 contributes to suspectibility to breast cancer and the malignancy of a subset of human ductal pancreatic cancer. Akt2 promotes metastasis of tumour cells, but does not affect the latency of tumour development. Together with Akt3, it has a critical role in glioblastomas. However, there are no prominent mutations in Akt2 in human cancers, with a relatively dispersed pattern of low level mutations through out the full length of the protein. In cancer, it is found to be overexpressed and amplified. It is also linked with invasion, cell migration and induction of angiogenesis. This description may include information annotated by UniProt and/or Google AI.