Product Name:
PAK6-APBD
Product Number:
ab-nk124-2
Target Full Name: Protein-serine/threonine kinase PAK 6
Target Alias: PAK5; KIAA1264; P21 protein (Cdc42/Rac)-activated kinase 7; P21-activated kinase 7; PAK 7; PAK-5; PAK7; PAK-7; MGC26232; RP5-1119D9_3; ENSG00000101349; KIAA1264KIAA1264; P21 protein (Cdc42/Rac)-activated kinase 7; P21-activated kinase 7; PAK 7; PAK-5; PAK7; PAK-7; MGC26232; RP5-1119D9_3; ENSG00000101349; KIAA1264
Product Type Specific: PAK6 protein kinase pan-specific antibody
Antibody Code: NK124-2
Antibody Target Type: Pan-specific
Protein UniProt: Q9NQU5
Protein SigNET: PAK6
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PAK6 sequence peptide Cat. No.: PE-01BEH90
Antibody Immunogen Sequence: CGSAMPVDGYISGLLNDIQK
Antibody Immunogen Description: Corresponds to amino acid residues G73 to K91; Post-PBD.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity of a target-sized protein by Western blotting in various mouse brain.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Chicken.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 70-74 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in diverse mouse tissues, except for a ~65 kDa protein in mouse testes, and weaker 50 and 37 kDa proteins in mouse brain, skeletal muscle and thymus. No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells, except for a strong 38 kDa proten in A431 cells.
Related Product 1: PAK6 (73-91) antibody blocking peptide (Cat. No.: PE-01BEH90)
Related Product 2: PAK4 S474 phosphorylation antibody (Cat. No.: AB-PK752)
Related Product 3: PAK5 expression antibody (Cat. No.: AB-NK190-2)
Related Product 4: PAK5 expression antibody (Cat. No.: AB-NK190-3)
Related Product 5: PAK5 S602 phosphorylation antibody (Cat. No.: AB-PK753)
Scientific Background: PAK6 (PAK5) is a protein-serine/threonine kinase of the STE group and STE20 family. It is primarily expressed in the testis and prostate, in the cytoplasm and nucleus. In some contexts, it acts as a regulator of neuronal, orneurite outgrowth. PAK6 binds to LRRK2 and is involved in neuron projection growth and potential mechanisms related to Parkinson's disease. It plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including the androgen receptor (AR) or MAP2K6/MAPKK6. PAK6 interacts with AR to regulate prostate cancer growth. It phosphorylates the DNA-binding domain of androgen receptor/AR and inhibits AR-mediated transcription. It also inhibits ESR1-mediated transcription. It may play a role in cytoskeleton regulation by interacting with IQGAP1. It can protect cells from apoptosis through phosphorylation of BAD. The activation of PAK6 through MAP2K6/MAPKK6 can be inhibited through the three mutations S165A, S560A, and Y566F. PAK6 expression is often increased in prostate cancer. PAK6 suppresses tumour progression through regulation of androgen receptor homeostasis. High expression of PAK6 is linked to tumor aggressiveness and poor prognosis in gastric cancer, while in other contexts it may act to suppress prostate tumour growth. This description may include information annotated by UniProt and/or Google AI.