Product Name:
PAK1-NT (PAK1-1)
Product Number:
ab-nk122-2
Target Full Name: p21-activated kinase 1 alpha; Protein-serine/threonine kinase PAK 1
Target Alias: ADRB2; Alpha-PAK; CDC42,RAC effector kinase PAK-A; CDC42/RAC effector kinase PAK-A; Kinase PAK1; MUK2; P21 protein (Cdc42/Rac)-activated kinase 1; P65-PAK; STE20, yeast; P68-PAK; PAK 1; PAK-1; PAKA; Protein kinase MUK2; PAKalpha; MGC130000; MGC130001; CCDS8250.1; Q13153; ENSG00000149269
Product Type Specific: PAK1 protein kinase pan-specific antibody
Antibody Code: NK122-2
Antibody Target Type: Pan-specific
Protein UniProt: Q13153
Protein SigNET: PAK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PAK1 (PAKa) sequence peptide
Antibody Immunogen Sequence: MSNNGLDVQDKPC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to P12; N-terminus.
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 60-67 kDa.
Antibody Cross Reactivity: Cross-reactivity with 60 kDa and 90 kDa proteins in various rat tissues.
Related Product 1: PAK1-pS144 phosphosite-specific antibody (Cat. No.: AB-PK748)
Related Product 2: PAKtide KinSub kinase substrate peptide (Cat. No.: PE-01ADH95)
Related Product 3: PAK1Subtide kinase substrate peptide (Cat. No.: PE-01BIL95)
Related Product 4: PAK1 S144 phosphorylation antibody (Cat. No.: AB-PK748)
Related Product 5: PAK1 T423 phosphorylation antibody (Cat. No.: AB-PK749)
Related Product 6: PAK2 expression antibody (Cat. No.: AB-NK200)
Related Product 7: PAK2 S141 phosphorylation antibody (Cat. No.: AB-PK750)
Related Product 8: PAK2 Y130 phosphorylation antibody (Cat. No.: AB-PK751)
Scientific Background: PAK1 is a protein-serine/threonine kinase that is a member of the STE group of protein kinases in the STE20 family, and PAKA subfamily. This kinase is highly expressed and widely distributed in most tested human tissues. PAK1 has been implicated in the regulation of cytoskeletal organization, and cell proliferation and survival, morphology, motility and transformation. It features a C-terminal kinase (catalytic) domain, and an N-terminal regulatory region with a CDC42/RAC1 interactive-binding (CRIB) domain, which mediates its activation, and three proline-rich motifs that interact with adaptor proteins. Binding of GTP-bound Cdc42 or Rac1 to the autoregulatory region at the N-terminus of PAK1 releases monomers from the autoinhibited dimer, enables phosphorylation at Thr-423 and activation of its phosphotransferase activity. It is also activated by binding to GTP-bound Cdc42, independent of the phosphorylation state of Thr-423. Phosphorylation of Ser-144, Ser-149, and THr-423 increases PAK1's phosphotransferase activity, whereas phosphorylation of Thr-84 (by OXSR1) and Tyr-212 inhibits its phosphotransferase activity. Phosphorylation of Ser-21 inhibits binding of Nck and PIX. PAK1 may be a therapeutic target in B-Raf wild-type melanoma. It is overexpressed in breast, ovarian, and colorectal cancers, and promotes tumour growth and metastasis. Knockdown of PAK1 has been shown to inhibit the proliferation of mutant KRAS colon cancer cells. This description may include information annotated by UniProt and/or Google AI.