Product Name:
PAK6-APBD
Product Number:
ab-nk122-2
Target Full Name: Protein-serine/threonine kinase PAK 6
Target Alias: PAK5; KIAA1264; P21 protein (Cdc42/Rac)-activated kinase 7; P21-activated kinase 7; PAK 7; PAK-5; PAK7; PAK-7; MGC26232; RP5-1119D9_3; ENSG00000101349; KIAA1264KIAA1264; P21 protein (Cdc42/Rac)-activated kinase 7; P21-activated kinase 7; PAK 7; PAK-5; PAK7; PAK-7; MGC26232; RP5-1119D9_3; ENSG00000101349; KIAA1264
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK124-2
Antibody Target Type: Pan-specific
Protein UniProt: Q9NQU5
Protein SigNET: Q9NQU5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PAK6 sequence peptide Cat. No.: PE-01BEH90
Antibody Immunogen Sequence: CGSAMPVDGYISGLLNDIQK
Antibody Immunogen Description: Corresponds to amino acid residues G73 to K91; Post-PBD
Production Method: Corresponds to amino acid residues G73 to K91; Post-PBD
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity of a target-sized protein by Western blotting in various mouse brain.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 70-74 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in diverse mouse tissues, except for a ~65 kDa protein in mouse testes, and weaker 50 and 37 kDa proteins in mouse brain, skeletal muscle and thymus. No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells, except for a strong 38 kDa proten in A431 cells.
Related Product 1: PAK6-APBD blocking peptide
Scientific Background: PAK6 (PAK5) is a protein-serine/threonine kinase of the STE group and STE20 family. It can regulate gene transcription. MAPKK6/MAP2K6 or AR can induce PAK6 activity which will induce phosphorylation of the DNA-binding domain on an androgen receptor (AR) protein. The activation of PAK6 through MAP2K6/MAPKK6 can be inhibited through the three mutations S165A, S560A, and Y566F. It plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. It phosphorylates the DNA-binding domain of androgen receptor/AR and inhibits AR-mediated transcription. It also inhibits ESR1-mediated transcription. It may play a role in cytoskeleton regulation by interacting with IQGAP1. It can protect cells from apoptosis through phosphorylation of BAD. PAK6 suppresses tumour progression through regulation of androgen receptor homeostasis.