Product Name:
Mos (Mos-1)
Product Number:
ab-nk112
Target Full Name: Moloney sarcoma oncogene-encoded protein-serine kinase
Target Alias: ADRB2; C-mos; MGC119963; MSV; MGC119962; CCDS6164.1; ENSG00000172680
Product Type Specific: Mos protein kinase pan-specific antibody
Antibody Code: NK112
Antibody Target Type: Pan-specific
Protein UniProt: P00540
Protein SigNET: Mos
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Mos sequence peptide Cat. No.:
Antibody Immunogen Sequence: ASQRSFWAELNIARLRHDNIVRVVAASTRC
Antibody Immunogen Description: Corresponds to amino acid residues A101 to R129 of mouse Mos (Uniprot ID P00536); Kinase Catalytic Subdomain III.
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity on Western blots with recombinant Xenopus laevis Mos expressed as a glutathione S-transferase fusion protein.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Frog.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-45 kDa.
Antibody Cross Reactivity: Cross-reactivity with an unidentified rat protein of 60 kDa that is found in heart, liver and muscle.
Related Product 1: Mos-pY263 phosphosite-specific antibody (Cat. No.: AB-PK722)
Scientific Background: Mos is a protein-serine/threonine kinase of the Other group and Mos family. During oocyte maturation, it acts as a kinase that directly phosphorylates and activates MEK1 and MEK2 in the MAP kinase signalling module. High levels of Mos are found in germ cells. It is also known as "cytostatic factor" in unfertilized oocytes. It arrests meiosis at metaphase II, to ensure successful egg maturation. Mos is involved in meiotic spindle organization and chromatin organization. It is activated by phosphorylation at Ser-3, and inhibited by phosphorylation at Ser-102. Mos was originally identified as an oncoprotein from avial retroviral studies, where it was discovered as the transforming component (v-mos) of the Moloney murine sarcoma virus (Mo-MSV). It causes aggressive fibrosarcomas in animal models. In human health, alterations like aberrant DNA methylation of the MOS gene are linked to pre-cancerous tissue changes and the long-term development of gastric cancers. In certain cancers, such as lung adenocarcinomas, overexpressed Mos can completely substitute for missing or blocked upstream drivers like EGFR. It does this by activating both the MAPK and PI3K/AKT cell survival pathways. In somatic cells lacking functional p53, elevated Mos protein disrupts the normal coalescence of supernumerary centrosomes. This leads to the formation of multipolar mitotic spindles. The resulting faulty spindle assembly causes unequal chromosome distribution during division, driving gross genomic instability and aneuploidy. Mos is associated with Oocyte/Zygote/Embryo Maturation Arrest 20 and infertility. Female mice deficient in MOS (MOS -/-) show significantly reduced fertility because their oocytes fail to arrest properly at metaphase II. This description may include information annotated by UniProt and/or Google AI.