Product Name:
MEKK2-3
Product Number:
ab-nk108-5
Target Full Name: MAPK/ERK kinase kinase 2; Mitogen-activated protein kinase kinase kinase 2
Target Alias: Kinase MEKK2; M3K2; MAP/ERK kinase kinase 2; MAP3K2; MAPK/ERK kinase kinase 2; MAPKKK2; MEK kinase 2; MEKK 2; MEKK2B; ENSG00000169967
Product Type Specific: MEKK2 (MAP3K2) protein kinase pan-specific antibody
Antibody Code: NK108-5
Antibody Target Type: Pan-specific
Protein UniProt: Q9Y2U5
Protein SigNET: MEKK2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MEKK2 (MAP3K2) sequence peptide Cat. No.: PE-01AXV99
Antibody Immunogen Sequence: CKLRPSADELLRHMFV
Antibody Immunogen Description: Corresponds to amino acid residues K602 to V616; Kinase last alpha-chain.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Mouse | Chicken.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 66-74 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human MEKK6 and YSK4.
Related Product 1: MEKK2 (602-616) antibody blocking peptide (Cat. No.: PE-01AXV99)
Related Product 2: MEKK2-1 pan-specific antibody (Cat. No.: AB-NK108-3)
Related Product 3: MEKK2-2 pan-specific antibody (Cat. No.: AB-NK108-4)
Related Product 4: MEKK2-pS239 phosphosite-specific antibody (Cat. No.: AB-PK700)
Related Product 5: MEKK6-2 pan-specific antibody (Cat. No.: AB-NK225-2)
Related Product 6: MEKK6-3 pan-specific antibody (Cat. No.: AB-NK225-3)
Scientific Background: MEKK2 (MAP3K2) is a 70 kDa protein-serine/threonine kinase of the STE group and STE11 family. It functions as a critical MAP3K in signaling pathways regulating immune response, inflammation, and cell proliferation. It acts as an upstream kinase, primarily activating the JNK1/2, p38, ERK5, and NF-κB pathways, but It also binds to Protein Kinase C-related Kinase 2 (PRK2). It is required for cytokine production in cultured mast cells. The protein is one of the kinases that regulate different steps of the caveolar cycle. It features a C-terminal catalytic serine/threonine kinase domain and a distinct N-terminal regulatory domain. MEKK2 activity is regulated through 14-3-3 protein binding, which modulates dimerization. It is also activated by phosphorylation on Ser-514, Ser-520, and Thr-522. It is also known to form a complex with Mip1, which acts as an inhibitor. Methylation of the protein increases MAP kinase signalling and promotes Ras-driven carcinomas. Overexpression of MEKK2 has been associated with colorectal cancer progression, where it typically resides in the cytoplasm. This description may include information annotated by UniProt and/or Google AI.