Product Name:
MEKK-NT (MEKK1-1)
Product Number:
ab-nk106-5
Target Full Name: MAPK/ERK kinase kinase 1; Mitogen-activated protein kinase kinase kinase 1 [Fragment]
Target Alias: Kinase MEKK1; M3K1; MAP3K1; MAPK/ERK kinase kinase 1; MAPKKK1; MEK kinase 1; MEKK; MEKK1; Mitogen-activated protein kinase kinase kinase 1; Q13233; ENSG00000095015
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK107-3
Antibody Target Type: Pan-specific
Protein UniProt: Q13233
Protein SigNET: Q13233
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MEKK1 (MAP3K1) sequence peptide Cat. No.:
Antibody Immunogen Sequence: CGSTHFTRMRRRLMAIAD
Antibody Immunogen Description: Corresponds to amino acid residues G35 to D51; N-terminus
Production Method: Corresponds to amino acid residues G35 to D51; N-terminus
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with recombinant murine MEKK1 protein expressed in E. coli.
Antibody Species Reactivity: Human; Mouse; Rat
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 130-170 kDa.
Antibody Cross Reactivity: Cross-reactivity with ~78 kDa proteins in crude extracts from sea star oocytes., mouse oligodendrocytes, and sheep platelets.
Scientific Background: MEKK1 (MAP3K1) is a protein-serine/threonine kinase of the STE group and STE11 family. It can phosphorylate MEK1 (MAP2K1) and MKK4 (MAP2K4) leading to activation of ERK and JNK kinases and activation of CHUK and IKKb. It is ctivated by phosphorylation at T1400 and T1412. Phosphorylation of S67 inhibits its interaction with JNK1. MEKK1 may be a tumour suppressor protein in view of the higher frequency of deletions and insertions of the gene encoding this protein kinase in human tumours. Defects in MEKK1 have been linked with the rare condition 46xy Sex Reversal 3, which can include symptoms such as gonadal dysgenesis, testis abnormalities, and testicular cancer.
