Product Name:
MKK6-2
Product Number:
ab-nk105-4
Target Full Name: MAPK/ERK protein-serine kinase 6 (MKK6); Dual specificity mitogen-activated protein kinase kinase 6
Target Alias: Kinase MKK6; MAP kinase kinase 6; MAPK/ERK kinase 6; MAP2K6; MAPKK6; MKK6; Mitogen-activated protein kinase kinase 6; MP2K6; SAPKK3; CCDS11686.1; P52564; ENSG00000108984
Product Type Specific: MKK6 (MAP2K6) protein kinase pan-specific antibody
Antibody Code: NK105-4
Antibody Target Type: Pan-specific
Protein UniProt: P52564
Protein SigNET: MKK6
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MKK6 (MAP2K6, MEK6) sequence peptide Cat. No.: PE-01AWI99
Antibody Immunogen Sequence: CSKERPTYPELMQHPF
Antibody Immunogen Description: Corresponds to amino acid residues S299 to F313; Kinase last alpha-chain.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with recombinant human MKK6 on protein dot blots.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse | Platypus | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 37-43 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Weak immunoreactivity on protein dot blots with recombinant human MKK4, and no immunoreactivity with recombinant human MEK1 and MEK2.
Related Product 1: MEK6 (299-313) antibody blocking peptide (Cat. No.: PE-01AWI99)
Related Product 2: MKK3 expression antibody (Cat. No.: AB-NK101-4)
Related Product 3: MKK3 expression antibody (Cat. No.: AB-NK101-6)
Related Product 4: MKK3 S218 phosphorylation antibody (Cat. No.: AB-PK713)
Related Product 5: MKK3 Y230 phosphorylation antibody (Cat. No.: AB-PK714)
Related Product 6: MKK6 expression antibody (Cat. No.: AB-NK105-3)
Related Product 7: MKK6 expression antibody (Cat. No.: AB-NK105-4)
Scientific Background: MKK6 (MAP2K6, MEK6) is a 37 kDa Dual specificity protein kinase of the STE group and STE7 family. It selectively phosphorylates p38 MAPKs in response to inflammatory cytokines and environmental stresses, regulating crucial processes such as apoptosis, cell cycle arrest, and differentiation. It is critical for mediating inflammatory signalling, cardiomyocyte development, neutrophil migration, and T-cell activation. It can regulate expression of genes critical for survival of newly differentiated neurons. It is activated by dual phosphorylation on Ser-207 and Thr-211; S207E and T211E are linked with constitutive activation. With MAP3K3/MKK3, it catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the p38 MAP kinases MAPK11, MAPK12, MAPK13 and MAPK14, and it plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG-dependent gene transcription. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. It also acts as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases. MKK6 is implicated in cardiomyopathy and ischemia, as well as tumor suppressor roles in certain cancers. This description may include information annotated by UniProt and/or Google AI.