Product Name:
MKK7-2
Product Number:
ab-nk105-4
Target Full Name: MAPK/ERK protein-serine kinase 7 (MKK7); Dual specificity mitogen-activated protein kinase kinase 7
Target Alias: c-Jun N-terminal kinase kinase 2; JNK activating kinase 2; JNK kinase 2; JNK-activating kinase 2; JNKK2; Jun N-terminal kinase kinase 2; MAP2K7; MKK7; PRKMK7; O14733; ENSG00000076984
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK106-4
Antibody Target Type: Pan-specific
Protein UniProt: O14733
Protein SigNET: O14733
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MKK7 (MEK7, MAP2K7) sequence peptide Cat. No.: PE-01AWK99
Antibody Immunogen Sequence: CKYNKLLEHSFIKRYE
Antibody Immunogen Description: Corresponds to amino acid residues K370 to E384; Kinase last alpha-chain
Production Method: Corresponds to amino acid residues K370 to E384; Kinase last alpha-chain
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 48-52 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human MEK2, MKK4 and MKK6.
Related Product 1: MKK7-2 blocking peptide
Related Product 2: MKK7 pan-specific antibody (Cat. No.: AB-NK106-5P)
Related Product 3: MKK7-3 pan-specific antibody (Cat. No.: AB-NK106-5)
Related Product 4: MKK7-pT275 phosphosite-specific antibody (Cat. No.: AB-PK717)
Scientific Background: MKK7 (MAP2K7, MEK7) is a dual-specificity protein kinase that functions in the MAP kinase (MAPK) and the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signalling pathways. It acts as an essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signalling pathway. It is activated by phosphorylation at S271 and T275 in the kinase activation loop by specific MAP kinase kinase kinases including MAP3K1 (MEKK1), MAP3K3 (MEKK3), MAP3K11 (MLK3) and MAP3K12 (DLK). With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. MEK7 has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signalling pathway is also involved in mitochondrial death signalling pathway, including the release cytochrome c, leading to apoptosis.
