Product Name:
MKK6-1
Product Number:
ab-nk104-4
Target Full Name: MAPK/ERK protein-serine kinase 6 (MKK6); Dual specificity mitogen-activated protein kinase kinase 6
Target Alias: Kinase MKK6; MAP kinase kinase 6; MAPK/ERK kinase 6; MAP2K6; MAPKK6; MKK6; Mitogen-activated protein kinase kinase 6; MP2K6; SAPKK3; CCDS11686.1; P52564; ENSG00000108984
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK105-3
Antibody Target Type: Pan-specific
Protein UniProt: P52564
Protein SigNET: P52564
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MKK6 (MAP2K6, MEK6) sequence peptide Cat. No.: PE-01AWH90
Antibody Immunogen Sequence: CISIGNQNFEVKADDL
Antibody Immunogen Description: Corresponds to amino acid residues S40 to L53
Production Method: Corresponds to amino acid residues S40 to L53
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with recombinant human MKK6 on protein dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 37-43 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: Medium immunoreactivity on protein dot blots with recombinant human MKK4, and no immunoreactivity with MEK1 and MEK2.
Related Product 1: MKK6-1 blocking peptide
Related Product 2: MKK6-2 pan-specific antibody (Cat. No.: AB-NK105-4)
Related Product 3: MKK6-4 pan-specific antibody (Cat. No.: AB-NK105-5)
Scientific Background: MKK6 (MAP2K6, MEK6) is a Dual specificity protein kinase of the STE group and STE7 family. It can regulate expression of genes critical for survival of newly differentiated neurons. MKK6 is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. It is probably activated by dual phosphorylation on S207 and T211. S207E and T211E are linked with constitutive activation. With MAP3K3/MKK3, it catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the p38 MAP kinases MAPK11, MAPK12, MAPK13 and MAPK14, and it plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG-dependent gene transcription. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. It also acts as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases.
