Product Name:
MEK5-PNT (MEK5-4)
Product Number:
ab-nk104-2
Target Full Name: MAPK/ERK protein-serine kinase 5 (MEK5); Dual specificity mitogen-activated protein kinase kinase 5
Target Alias: CXA1; HsT17454; MAP kinase kinase 5; MAP2K5; MAPK,ERK kinase 5; MAPK/ERK kinase 5; MAPKK5; MKK5; PRKMK5; HsT17454; ENSG00000137764
Product Type Specific: MEK5 (MAP2K5) rotein kinase pan-specific antibody
Antibody Code: NK104-2
Antibody Target Type: Pan-specific
Protein UniProt: Q13163
Protein SigNET: MEK5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MEK5 (MAP2K5, MKK5) sequence peptide
Antibody Immunogen Sequence: AFEYEDEDGDRITVRSC
Antibody Immunogen Description: Corresponds to amino acid residues A59 to S74; Post-N-terminus.
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Rat | Mouse | Frog.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 47-52 kDa.
Related Product 1: MEK5-1 pan-specific antibody (Cat. No.: AB-NK104-3)
Related Product 2: MEK5-2 pan-specific antibody (Cat. No.: AB-NK104-4)
Related Product 3: MEK5-3 pan-specific antibody (Cat. No.: AB-NK104-5)
Related Product 4: MEK5-pS311 phosphosite-specific antibody (Cat. No.: AB-PK699)
Scientific Background: MEK5 (MAP2K5) is a Dual specificity protein kinase of the STE group and STE7 family. It is essential for early embryogenesis, cell survival, angiogenesis, cardiovascular development, and vascular function. It is activated by phosphorylation at Ser-311 and Thr-315 by MAP kinase kinase kinases such as MAP3K2/3 (MEKK2/3) or atypical protein kinase C (aPKC) isoforms. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis by acting as a scaffold protein for the construction of a complex consisting of MAP3K2 or MAP3K3 and MAP3K5 and MAPK7. Alternative splicing produces two isoforms, with MEK5-alpha containing a N-terminal PB1 (phox and Bem1p) domain that binds to ERK5 and acts as a scaffold for the signalling complex, which is essential for mediating interactions with upstream activators. Overexpression and overactivation of the MEK5/ERK5 pathway are associated with various cancers (e.g., prostate cancer), promoting tumour growth and chemotherapy resistance. This description may include information annotated by UniProt and/or Google AI.