Product Name:
MEK2-NT (MEK2-4)
Product Number:
ab-nk099-7
Target Full Name: MAPK/ERK protein-serine kinase 2 (MKK2); Dual specificity mitogen-activated protein kinase kinase 2
Target Alias: ERK activator kinase 2; MAP kinase kinase 2; MAP2K2; MAPK,ERK kinase 2; MAPKK2; MKK2; MP2K2; PRKMK2EC; MEK2; PRKMK2; CCDS12120.1; ENSG00000126934
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK100-3
Antibody Target Type: Pan-specific
Protein UniProt: P36507
Protein SigNET: P36507
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MEK2 (MKK2, MAP2K2) sequence peptide
Antibody Immunogen Sequence: MLARRKPVLPALTC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to T13; N-terminus
Production Method: Corresponds to amino acid residues M1 to T13; N-terminus
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with human and mouse MEK2 in human cell lines and mouse tissues. Strong immunoreactivity on Western blots with recombinant rat MEK2 expressed in COS cells.
Antibody Species Reactivity: Human. Mouse; Rat
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 43-50 kDa.
Related Product 2: MEK2 pan-specific antibody (Cat. No.: AB-NK100-4P)
Related Product 3: MEK2-1 pan-specific antibody (Cat. No.: AB-NK100-5)
Related Product 4: MEK2-2 pan-specific antibody (Cat. No.: AB-NK100-4)
Related Product 5: MEK2-3 pan-specific antibody (Cat. No.: AB-NK100-6)
Related Product 6: MEKSubtide - MEK1 (MAP2K1) protein kinase substrate peptide
Scientific Background: MEK2 (MAP2K2) is a Dual specificity protein kinase of the STE group and STE7 family. Phosphorylation of S222 and S226 increases its phosphotransferase activity. Phosphorylation of S216 inhibits phosphotransferase activity. It controls the concomitant phosphorylation of the threonine and tyrosine in a Thr-Glu-Tyr sequence in MAP kinases. It particularly targets and activates the ERK1 and ERK2 MAP kinases.
