Product Name:
Ksr1
Product Number:
ab-nk090
Target Full Name: Protein-serine kinase suppressor of Ras 1
Target Alias: CAP kinase; Ceramide-activated protein kinase; HB; HB protein; Ksr; Ksr1; Kinase suppressor of ras 1; Kinase suppressor of RAS-1; RSU2; ENSG00000141068
Product Type Specific: KSR1 protein kinase pan-specific antibody
Antibody Code: NK090
Antibody Target Type: Pan-specific
Protein UniProt: Q8IVT5
Protein SigNET: Ksr1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Ksr1 sequence peptide
Antibody Immunogen Sequence: EKLPKLNRRLSHPGHFWKSC
Antibody Immunogen Description: Corresponds to amino acid residues E878 to S896; C-terminus.
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 105-120 kDa.
Related Product 1: Ksr1-pS406 phosphosite-specific antibody (Cat. No.: AB-PK675)
Related Product 2: Ksr2-pS490 phosphosite-specific antibody (Cat. No.: AB-PK676)
Scientific Background: Ksr1 (kinase suppressor of Ras 1) is a 90-100 kDa protein-serine/threonine kinase of the TKL group and RAF family. It is a Raf-related pseudokinase and may directly mediate Raf activation. It acts as a scaffold for the Raf/MEK/ERK kinase cascade. It features five conserved domains (CA1–CA5) and functions mainly as a homodimer, with structural similarities to RAF kinases. It was originally described as a "kinase suppressor" in Drosophila, where its loss suppressed activated Ras mutations. While it possesses a kinase-like domain, its primary function is as a scaffold. Its direct ATP-binding ability is considered low or controversial, but it acts as an allosteric activator of BRAF and facilitates MEK phosphorylation. It resides in the cytoplasm in resting cells and translocates to the plasma membrane upon receptor activation. No specific mutations have been directly implicated in oncogenesis although loss of Ksr1 function will blunt the Ras pathway and therefore attenuate its oncogenic potential. Elevated KSR1 levels is linked to tumour proliferation (e.g., breast cancer, colon cancer). This description may include information annotated by UniProt and/or Google AI.