Product Name:
TAOK3-AKCD
Product Number:
ab-nk087-2
Target Full Name: Serine/threonine-protein kinase TAO3
Target Alias: DPK; JIK; Kinase TAO3; MAP3K18; TAO kinase 3; TAOK3
Product Type Specific: TAO3 (TAOK3) protein kinase pan-specific antibody
Antibody Code: NK087-2
Antibody Target Type: Pan-specific
Protein UniProt: Q9H2K8
Protein SigNET: TAOK3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TAOK3 (JIK, TAO3) sequence peptide Cat. No.: PE-01BFJ
Antibody Immunogen Sequence: CSEHGTSLNREMDSLGSNHS
Antibody Immunogen Description: Corresponds to amino acid residues S331 to S349; After catalytic domain.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.43 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Chicken | Frog.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 115-125 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactivities detected in mouse brain, skeletal muscle, testes and thymus, or in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells.
Related Product 1: TAO3 (331-349) antibody blocking peptide (Cat. No.: PE-01ACY01)
Related Product 2: TAOSubtide kinase substrate peptide (Cat. No.: PE-01BJN95)
Related Product 3: TAO1-pS181 phosphosite-specific antibody (Cat. No.: AB-PK826)
Related Product 4: TAO1-pY309 phosphosite-specific antibody (Cat. No.: AB-PK827)
Scientific Background: TAOk3 (Thousand-and-one amino acid kinase 3; MAP3K18, TAO3) is a protein-serine/threonine kinase of the STE group and STE20 family. It is a cutaneous T-cell lymphoma-associated antigen. It function as a MAP kinase kinase kinase (MAP3K) in mammals, playing crucial roles in immune cell regulation, osteoblast differentiation, dendritic cell terminal differentiation, and cellular stress responses. TAOK3 regulates p38 and JNK cascades, contributes to ADAM10-mediated B-cell development. It also functions in the G2-M transition in response to DNA damage. TAOK3 is found at the cell membrane (membrane rafts), in the cytoplasm, and is associated with lipid droplets, acting as a peripheral membrane protein. Specific single-nucleotide polymorphisms (SNPs) in the TAOK3 gene have been associated with higher opioid requirements for pain management in advanced cancer patients. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis. TAOK3 is involved in cancer progression (e.g., melanoma, breast, bladder) by aiding in invadopodia formation and endosomal trafficking of the scaffold protein TKS5α. This description may include information annotated by UniProt and/or Google AI.