Product Name:
InsR-BCT
Product Number:
ab-nk076-6
Target Full Name: Insulin receptor
Target Alias: CD220; CD220 antigen; HHF5; Insulin receptor; IR; Kinase InsR
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK079-2
Antibody Target Type: Pan-specific
Protein UniProt: P06213
Protein SigNET: P06213
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human InsR (IR) sequence peptide Cat. No.: PE-01BDI85
Antibody Immunogen Sequence: SLGFKRSYEEHIPYTHMNGC
Antibody Immunogen Description: Corresponds to amino acid residues S1348 to G1366; Pre-C-terminus
Production Method: Corresponds to amino acid residues S1348 to G1366; Pre-C-terminus
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 90-100 kDa.
Related Product 1: InsR-BCT blocking peptide
Related Product 2: InsR-pY1189 phosphosite-specific antibody (Cat. No.: AB-PK663)
Related Product 3: InsRSelectide - Insulin receptor protein kinase substrate peptide
Related Product 4: InsRSubtide - InsR protein kinase substrate peptide
Related Product 5: IRS1 (979-989) KinSub - Insulin receptor substrate 1 (K979-G989, mouse) peptide; Insulin receptor substrate
Scientific Background: InsR (IR) is a protein-tyrosine kinase of the TK group and InsR family. It binds and mediates the effects of insulin. Binding of insulin to the receptor results in the downstream phosphorylation of several intracellular signalling components, including insulin receptor substrates (IRS1, 2, 3, 4), Shc, GAB1, CBL, and other intracellular signalling intermediates. Phosphorylated IRS1 recruits and activates the GRB2/SOS complex, which in turn leads to the activation of the Ras/Raf/MAP2K/MAPK signalling pathway. This pathway is mainly involved in the regulation of cell growth, survival, and cellular differentiation resulting from insulin signalling. Insulin binds to InsR to trigger autophosphorylation at many sites, including Y999, S1001, Y1185, Y1189, Y1190, S1332, S1333, Y1355, Y1361 and T1375, which increases its phosphotransferase activity. Protein interactions are induced with phosphorylation of Y999 (with SOCS3) and Y1185, Y1189 and Y1190 (with IRS2 and PTP1B). Phosphorylation of Y992 and Y1011 inhibits the phosphotransferase activity of InsR. Binding of insulin to the InsR stimulates glucose uptake. Insulin receptor signalling helps to maintain fuel homeostasis and prevent diabetes. InsR signalling also increases the regeneration of adult beta-cells and the central control of nutrient homeostasis. InsR has also been linked with the development of insulin resistance, familial hyperinsulinemic hypoglycemia Type 5 (HHF5), and gastric adenocarcinomas. InsR appears to be an oncoprotein (OP). Over activation of the insulin receptor signalling pathway has been linked to the development of several cancer types, including breast cancer. In addition, elevated InsR expression in breast cancer is correlated with poor patient survival.
