Product Name:
IKKb-NT
Product Number:
ab-nk076-6
Target Full Name: Inhibitor of nuclear factor kappa B kinase beta subunit
Target Alias: I-kappa-B kinase 2; I-kappa-B-kinase beta; IkBKB; IKK2; IKKB; IKK-beta; NFKBIKB; IKBKINASE
Product Type Specific: IKK2 protein kinase pan-specific antibody
Antibody Code: NK076-6
Antibody Target Type: Pan-specific
Protein UniProt: O14920
Protein SigNET: IKKb
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human IKKb (IkBKB) sequence peptide Cat. No.: PE-01BDH90
Antibody Immunogen Sequence: MSWSPSLTTQTSGAWEMKEC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to E19; N-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 87-97 kDa.
Related Product 1: IKKb (1-19) antibody blocking peptide (Cat. No.: PE-01BDH90)
Related Product 2: IKKSelectide kinase substrate peptide (Cat. No.: PE-01BHN95)
Related Product 3: IKKa-pT179+pS180 phosphosite-specific antibody (Cat. No.: AB-PK659)
Related Product 4: IKKb-BCT pan-specific antibody (Cat. No.: AB-NK076-5)
Related Product 5: IKKe-pS172 phosphosite-specific antibody (Cat. No.: AB-PK660)
Related Product 6: IKKg S377 phosphorylation antibody (Cat. No.: AB-PN758)
Scientific Background: IKKb (IKBKB) is an 87 kDa protein-serine/threonine kinase of the Other group and IKK family. It functions in NF-kappa-B signalling and is activated by various indicators of cellular stress, including inflammatory cytokines, bacterial or viral products, and DNA damage. It is a key component of the IKK complex, with IKK-alpha and the regulatory subunit NEMO (IKK-gamma), which initiates the canonical pathway by phosphorylating IkappaB inhibitors, marking them for degradation and allowing NF-kappa-B activation. It is activated by phosphorylation on Ser-177 and Ser-181. It phosphorylates IκB (at Ser-32/36), leading to its polyubiquitination and proteasomal degradation, which allows the NF-κB transcription factor to translocate to the nucleus and stimulate the transcription of genes involved in the immune response, control of cell growth, and cellular protection against apoptosis. Beyond IκB, IKKb phosphorylates NEMO, NF-κB subunits (RELA, NFKB1), and the pro-apoptotic factor FOXO3, mediating its inactivation. Overactivation of IKKb is linked to chronic inflammation. IKKb may be a tumour requiring protein (TRP), since it displays extremely low rates of mutation in human cancers. This description may include information annotated by UniProt and/or Google AI.