Product Name:
GSK-3Beta
Product Number:
ab-nk070-4
Target Full Name: Glycogen synthase kinase-3 beta
Target Alias: GSK 3 beta; GSK 3B; GSK-3 beta; GSK3B; GSK3B_Human; GSK3beta isoform
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK070-4
Antibody Target Type: Pan-specific
Protein UniProt: P49841
Protein SigNET: GSK3b
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide corresponding to the sequence near the C-terminus of human GSK-3Beta
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1.3 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:2000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~47 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Cow | Rat | Mouse | Cow.
Antibody Positive Control: 1 µg/ml of SPC-169 was sufficient for detection of GSK-beta in 20 µg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Medium
Related Product 1: GSK3a expression antibody (Cat. No.: AB-NK069-3)
Related Product 2: GSK3a S278+Y279 phosphorylation antibody (Cat. No.: AB-PK647)
Related Product 3: GSK3a T19+S21 phosphorylation antibody (Cat. No.: AB-PK648)
Related Product 4: GSK3a Y279 phosphorylation antibody (Cat. No.: AB-PK649)
Related Product 5: GSK3a Y284+Y285 phosphorylation antibody (Cat. No.: AB-PK650)
Related Product 6: GSK3a Y279 phosphorylation antibody (Cat. No.: AB-PK882)
Related Product 7: GSK3b expression antibody (Cat. No.: AB-NK070)
Related Product 8: GSK3b expression antibody (Cat. No.: AB-NK270-3)
Related Product 9: GSK3b T275+T277 phosphorylation antibody (Cat. No.: AB-PK883)
Related Product 10: GSK3b T390 phosphorylation antibody (Cat. No.: AB-PK932)
Scientific Background: Glycogen synthase kinase 3 (GSK3) are serine/threonine protein kinases of the CMGC group and GSK family. They have been shown to regulate immune and migratory processes, and are tied to pathways of cell proliferation and apoptosis (1-3). In mammals, GSK3 is encoded by two related genes: GSK3-alpha and GSK3-beta, and they share about 85% amino acid identity. They are structurally similar, but functionally non-identical. GSK3-beta features a 7-strand-barrel N-terminal domain, a central kinase domain, and a C-terminal domain, with the active site located between the N-terminal and alpha-helical domains. GSK3-alpha and GSK3-beta are inhibited by phosphorylation at Ser-21 and Ser-9, respectively, by Akt1 and other kinases. They are activated by tyrosine phosphorylation (Tyr-279 in GSK3-alpha; Tyr-216 in GSK3-beta). GSK3-beta phosphorylates glycogen synthase (inactivating it) and regulates Wnt, PI3K/Akt, and MAPK-dependent pathways. GSK3-beta negatively regulates cardiac hypertrophy and cardiac development through its effect on WNT signalling (4). It is part of the beta-catenin destruction complex in the Wnt signaling pathway; it phosphorylates beta-catenin leading to its destruction. Both are widely expressed, especially in the brain and testis, and found in the cytoplasm and nucleus. GSK3-alpha and GSK3-beta have been implicated in a number of diseases including Type II diabetes, Alzheimer’s (tau phosphorylation), Parkinson's, bipolar, schizophrenia, and cancer (4, 5). This description may include information annotated by UniProt and/or Google AI.