Product Name:
p38g-1
Product Number:
ab-nk059-3
Target Full Name: Mitogen-activated protein-serine kinase p38 gamma; Mitogen-activated protein kinase 12
Target Alias: ERK3; ERK5; ERK6; MAPK12; MK12; p38 MAPK-gamma; PRKM12; SAPK3; Stress-activated protein kinase-3; CCDS14089.1; ENSG00000188130
Product Type Specific: p38-gamma protein kinase pan-specific antibody
Antibody Code: NK059-3
Antibody Target Type: Pan-specific
Protein UniProt: P53778
Protein SigNET: p38g
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human p38g MAPK (MAPK12, ERK6) sequence peptide Cat. No.: PE-01AYJ95
Antibody Immunogen Sequence: MSSPPPARSGFYRQEC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to E15; N-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Potency: Medium-strong immunoreactivity with recombinant human p38g on protein dot blots.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 38-42 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Medium-strong immunoreactivity on protein dot blots with p3b, very weak immunoreactivity with recombinant human p38d, and no immunoreactivity with p38a.
Related Product 1: p38g (1-15) antibody blocking peptide (Cat. No.: PE-01AYJ95)
Related Product 2: p38SelectideA kinase substrate peptide (Cat. No.: PE-14CAC90)
Related Product 3: p38Subtide kinase substrate peptide (Cat. No.: PE-01BIK99)
Related Product 4: p38g expression antibody (Cat. No.: AB-NK059-3)
Related Product 5: p38g expression antibody (Cat. No.: AB-NK059-4)
Related Product 6: p38g expression antibody (Cat. No.: AB-NK059-5)
Scientific Background: p38g (MAPK12, ERK6) is a protein-serine/threonine kinase of the CMGC group and p38 MAPK family. This kinase is moderate to highly expressed in most tested human tissues except in the pituitary, spinal cord and vagina, where it is notably absent. p38g is activated by phosphorylation at Tyr-185 and probably Thr-183, by MKK3 (MAP2K3, MEK3) and MKK6 (MAP2K6, MEK6). p38g is activated in response to stress. It has been linked with the development of inflammation, autoimmunity, diabetes and cancer. In skeletal muscle metabolism, 38g functions as a signal transducer during myoblasts to myotubes differentiation. Enforced localization of p38g in the nucleus or cytoplasm markedly attenuates the ability of the kinase to induce cell cycle arrest in fibroblasts. p38g increases basal glucose uptake and decreases DNP- and contraction-stimulated glucose uptake, partially by affecting levels of glucose transporter expression in skeletal muscle. Unlike p38a or p38b, p38g has a unique C-terminal sequence (KETXL) that allows it to bind directly to PDZ domain-containing proteins, such as SAP97/hDlg and alpha1-syntrophin. It phosphorylates distinct substrates, including topoisomerase IIa and SAP97. p38g overexpression is linked with highly metastatic breast cancer cell lines, and its expression is associated with the metastatic states of breast tumour samples. The gene was found to be deregulated in uterine leiomyoma. Loss of function may be important to confer survival and inhibit apoptosis for tumour cells. This description may include information annotated by UniProt and/or Google AI.