Product Name:

p38g-2


Product Number:

ab-nk059-3

Price:

Regular price
$89.00
Regular price
Sale price
$89.00

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Target Full Name: Mitogen-activated protein-serine kinase p38 gamma; Mitogen-activated protein kinase 12

Target Alias: ERK3; ERK5; ERK6; MAPK12; MK12; p38 MAPK-gamma; PRKM12; SAPK3; Stress-activated protein kinase-3; CCDS14089.1; ENSG00000188130

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK059-4

Antibody Target Type: Pan-specific

Protein UniProt: P53778

Protein SigNET: P53778

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human p38g MAPK (MAPK12, ERK6) sequence peptide Cat. No.: PE-01AYK90

Antibody Immunogen Sequence: CVQKYDDSFDDVDRTL

Antibody Immunogen Description: Corresponds to amino acid residues V323 to L337; Post-kinase last alpha-chain

Production Method: Corresponds to amino acid residues V323 to L337; Post-kinase last alpha-chain

Antibody Modification: Protein kinase pan-specific antibody

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 1 µg/ml for immunoblotting

Antibody Potency: Medium-strong immunoreactivity with recombinant human p38g on protein dot blots.

Antibody Species Reactivity: Human; Mouse

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 38-42 kDa.

Antibody Specificity: Very high

Antibody Cross Reactivity: Medium-strong with recombinant human p38b, very weak immunoreactivity with p38d, and no immunoreactivity with p38a.

Scientific Background: p38g (MAPK12, ERK6) is a protein-serine/threonine kinase of the CMGC group and p38 MAPK family. This kinase is moderate to highly expressed in most tested human tissues except in the pituitary, spinal cord and vagina, where it is notably absent. p38g is activated by phosphorylation at Y185 and probably T183. p38g is activated in response to stress. It has been linked with the development of inflammation, autoimmunity, diabetes and cancer. It functions as a signal transducer during myoblasts to myotubes differentiation. Enforced localization of p38g in the nucleus or cytoplasm markedly attenuates the ability of the kinase to induce cell cycle arrest in fibroblasts. p38g increases basal glucose uptake and decreases DNP- and contraction-stimulated glucose uptake, partially by affecting levels of glucose transporter expression in skeletal muscle. p38g overexpression is linked with highly metastatic breast cancer cell lines, and its expression is associated with the metastatic states of breast tumour samples. The gene was found to be deregulated in uterine leiomyoma. Loss of function may be important to confer survival and inhibit apoptosis for tumour cells.