Product Name:
PRKDC-4 (DNAPK)
Product Number:
ab-nk048-6
Target Full Name: DNA-dependent protein kinase catalytic subunit
Target Alias: DNA-PK catalytic subunit; DNA-PKcs; DNPK1; P460; PRKD; PRKDC; XRCC7; HYRC; HYRC1; p350; ENSG00000121031
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK048-7
Antibody Target Type: Pan-specific
Protein UniProt: P78527
Protein SigNET: P78527
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DNAPK (PRKDC) sequence peptide Cat. No.: PE-01ARO85
Antibody Immunogen Sequence: CLMDQATDPNILGRTW
Antibody Immunogen Description: Corresponds to amino acid residues C4106 to W4121; C-terminus
Production Method: Corresponds to amino acid residues C4106 to W4121; C-terminus
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 460-480 kDa.
Related Product 1: PRKDC-4 (DNAPK) blocking peptide
Related Product 2: PRKDC-1 (DNAPK) pan-specific antibody (Cat. No.: AB-NK048-4)
Related Product 3: PRKDC-2 (DNAPK) pan-specific antibody (Cat. No.: AB-NK048-5)
Related Product 4: PRKDC-3 (DNAPK) pan-specific antibody (Cat. No.: AB-NK048-6)
Scientific Background: DNAPK (PRKDC) is a protein-serine/threonine kinase of the Atypical group and PIKK family. Phosphorylation at -612, T2609, T2638, T2647 and T3950 inhibits phosphotransferase activity. DNAPK has been noted to be involved in sensing DNA damage, including DNA non-homologous end joining (NHEJ) and therefore, by association, be involved with V(D)J recombination events and double-strand break (DSB) repair. A possible scaffolding role of DNAPK for DNA-repair enzymes has also been proposed. DNAPK appears to be a tumour suppressor protein (TSP). The active form of the protein kinase normally acts to inhibit tumour cell proliferation until damage to DNA can be repaired. Therefore, it can play a role to facilitate tumour cell survival under conditions of radiation and chemotherapy.