Product Name:
CK2a1-ANT
Product Number:
ab-nk041-2
Target Full Name: Casein protein-serine kinase 2 alpha/ alpha prime
Target Alias: Casein kinase 2, alpha 1 polypeptide; Casein kinase II, alpha chain; CK II; CK2-alpha1; CKIIA; CSK21; CSNK2A1; Kinase CK2-alpha1;
Product Type Specific: CK2A protein kinase pan-specific antibody
Antibody Code: NK041-2
Antibody Target Type: Pan-specific
Protein UniProt: P68400
Protein SigNET: CK2a1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CK2a1 (CSNK2A1) sequence peptide Cat. No.: PE-01BCP75
Antibody Immunogen Sequence: CARVYTDVNTHRPREYWDYE
Antibody Immunogen Description: Corresponds to amino acid residues A9 to E27; Post-N-terminus, conserved in both CK2 isoforms.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish | Fruit fly | Honey bee | Maize | Thale cress | .
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 44-47 kDa.
Related Product 1: CK2a1 (9-27) antibody blocking peptide (Cat. No.: PE-01BCP75)
Related Product 2: CK2Selectide - CK2a1 (CSNK2A1) protein kinase substrate peptide
Related Product 3: HSP90a (255-268) KinSub kinase substrate peptide (Cat. No.: PE-01ABK95)
Related Product 4: CK2pSelectide antibody blocking peptide (Cat. No.: PE-04AXK75)
Related Product 5: CK2a Y188 phosphorylation antibody (Cat. No.: AB-PK962)
Related Product 6: CK2a CK2a-pS194+pY196 phosphorylation antibody (Cat. No.: AB-PK963)
Related Product 7: CK2a Y255 phosphorylation antibody (Cat. No.: AB-PK582)
Related Product 8: CK2a T360+pS362 phosphorylation antibody (Cat. No.: AB-PK964)
Scientific Background: CK2a1 (CSNK2A1) is a 42 kDa protein-serine/threonine kinase of the Other group and CK2 family. CK2a1 is activated by the CK2-beta regulatory subunit in a complex with two alpha catalytic subunits and two beta subunits. It is also activated through phosphorylation near its C-terminus by p38a MAPK. CK2 is potently inhibited by heparin. It is involved in various cellular processes, such as cell cycle control, apoptosis, transcription, and viral infection. A unique feature of CK2 is its ability to use both ATP and GTP as phosphoryl donors. It can phosphorylate human p53 protein at Ser-392 with presence of UV irradiation, which may be a potential mechanism for p53 activation by UV irradiation. It also phosphorylates the caspases CASP9 and CASP2, and hence negatively regulates apoptosis. CK2a1 is an essential regulator of Mitochondria-Associated ER Membranes (MAMs). It phosphorylates PACS2 (at Ser-207/208/213) to manage MAM structure, Ca2+ homeostasis, and mitochondrial ATP production. It regulates numerous transcription factors (e.g., NF-κB, STAT1, MYC) and phosphorylates RNA polymerases I, II, III, and IV, facilitating transcription. CK2a1 protects cells by regulating p53 and phosphorylating caspases (CASP9, CASP2) to inhibit apoptosis. It also helps repair DNA single-strand breaks. It is utilized by viruses (e.g., SARS-CoV-2, HIV, HBV) to promote their own replication. CK2a1 levels are often up-regulated by 2-fold in expression in many human tumours, including gastric, lung, breast, and lymphoma. In Okur-Chung Neurodevelopmental Syndrome (OCNDS) there is inherited loss-of-function variants in the CSNK2A1 gene, and this condition is characterized by developmental delay, intellectual disability, and speech issues. This description may include information annotated by UniProt and/or Google AI.