Product Name:
CDK8-NT (CDK8-1)
Product Number:
ab-nk031-4
Target Full Name: Cyclin-dependent protein-serine kinase 8
Target Alias: Kinase CDK8; Protein kinase K35
Product Type Specific: CDK8 protein kinase pan-specific antibody
Antibody Code: NK031-4
Antibody Target Type: Pan-specific
Protein UniProt: P49336
Protein SigNET: CDK8
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDK8 sequence peptide
Antibody Immunogen Sequence: MDYDFKVKLSSERERC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to R15; N-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Mouse | Platypus | Chicken | Frog | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-60 kDa.
Antibody Cross Reactivity: Cross-reacts with an unidentified 69 kDa protein in various rat tissues.
Scientific Background: CDK8 is a protein-serine/threonine kinase of the CMGC group and CDK4 family. It is a component of the Mediator complex (along with Cyclin C, MED12 and MED13), which is a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. This complex functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. It phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II) to inhibit the formation of a transcription initiation complex. It phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. It is also recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, and promote its degradation. It plays a significant role in promoting transcriptional elongation of genes, including those involved in the serum response network and hypoxia-induced factor 1-alpha (HIF1a) target genes. CDK8 can interact with the tumour suppressor p53 to facilitate its function, indicating a complex, context-dependent role in tumour development. CDK8 is frequently overexpressed in colorectal cancer, promoting b-catenin-mediated transcription and driving tumour growth. This description may include information annotated by UniProt and/or Google AI.