Product Name:
CDK8-NT (CDK8-1)
Product Number:
ab-nk030-1
Target Full Name: Cyclin-dependent protein-serine kinase 8
Target Alias: Kinase CDK8; Protein kinase K35
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK031-4
Antibody Target Type: Pan-specific
Protein UniProt: P49336
Protein SigNET: P49336
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDK8 sequence peptide
Antibody Immunogen Sequence: MDYDFKVKLSSERERC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to R15; N-terminus
Production Method: Corresponds to amino acid residues M1 to R15; N-terminus
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Rat
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-60 kDa.
Antibody Cross Reactivity: Cross-reacts with an unidentified 69 kDa protein in various rat tissues.
Related Product 1: RNA polymerase II-CT KinSub - heptapeptide repeat from C-terminus peptide; CDKtide protein kinase substrate peptide
Scientific Background: CDK8 is a protein-serine/threonine kinase of the CMGC group and CDK4 family. It is a component of the Mediator complex, which is a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. This complex functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. It phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II) to inhibit the formation of a transcription initiation complex. It phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. It is also recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, and promote its degradation.