Product Name:
CaMK2g-PAD
Product Number:
ab-nk020-1
Target Full Name: Calcium/calmodulin-dependent protein-serine kinase 2 gamma
Target Alias: Calcium/calmodulin-dependent protein kinase II gamma; CaM kinase II gamma subunit; CaMK2-gamma; CaMKG; CaMK-II gamma; CaMK-II gamma subunit; CaM-kinase II gamma; CaM-kinase II gamma chain; EC 2.7.11.17; Kinase CaMK2-gamma
Product Type Specific: CaMK2g protein kinase pan-specific antibody
Antibody Code: NK020-1
Antibody Target Type: Pan-specific
Protein UniProt: Q13555
Protein SigNET: CaMK2g
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CaMK2g sequence peptide Cat. No.: PE-01BCJ90
Antibody Immunogen Sequence: CDGIKGSTESSNTTTEDEDL
Antibody Immunogen Description: Corresponds to amino acid residues D376 to L394; Before CaMAD, highly conserved in all CaMK2 isoforms.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-60 kDa.
Related Product 1: CaMK2g (376-394) antibody blocking peptide (Cat. No.: PE-01BCJ90)
Related Product 2: CaMK2a T286 phosphorylation antibody (Cat. No.: AB-PK555)
Related Product 3: CaMK2a Y230 phosphorylation antibody (Cat. No.: AB-PK875)
Related Product 4: CaMKII (Alpha expression antibody (Cat. No.: AB-NK302-1)
Related Product 5: CaMKII pT286/pT287 T286 phosphorylation antibody (Cat. No.: AB-NK302-2)
Related Product 6: CaMK2b expression antibody (Cat. No.: AB-NK018-3)
Related Product 7: CaMK2d expression antibody (Cat. No.: AB-NK019-3)
Scientific Background: CaMK2g is a protein-serine/threonine kinase of the CaMK group and CaMK2 family. It is a calcium/calmodulin-activated kinase that is involved in sarcoplasmic reticulum calcium transport, and may have a role in synaptic plasticity. Once activated by calcium or calmodulin CaMK2g will autophosphorylate at Thr-287, and become activate in the absence of calcium. It mediates autonomous regulation of neuronal dendritic spine formation, sarcoplasmic reticulum transport, and leukemic cell proliferation. It is involved in the JAK-STAT signaling pathway by phosphorylating STAT1, and plays a role in hippocampal-dependent learning and synaptic plasticity. It is highly expressed in the brain, heart, and muscle tissue. It is primarily found in the postsynaptic density (PSD) of neurons and the cytoplasm, but also within the plasma membrane and nucleus. CaMK2g resides in large multi-oligomeric protein complexes (12 subunits) with other CaMK2 subunits (alpha, beta, and delta). It is implicated in autosomal dominant intellectual developmental disorder 59 (MRD59). It is studied as a factor in cancer cell proliferation and vascular smooth muscle migration. This description may include information annotated by UniProt and/or Google AI.