Product Name:
MEKK2-pS239
Product Number:
ab-pk700
Target Full Name: MAPK/ERK kinase kinase 2; Mitogen-activated protein kinase kinase kinase 2
Target Alias: Kinase MEKK2; M3K2; MAP/ERK kinase kinase 2; MAP3K2; MAPK/ERK kinase kinase 2; MAPKKK2; MEK kinase 2; MEKK 2; MEKK2B; ENSG00000169967
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK700
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S239
Protein UniProt: Q9Y2U5
Protein SigNET: Q9Y2U5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MEKK2 (MAP3K2) sequence peptide Cat. No.: PE-04ANX90
Antibody Immunogen Sequence: RAQ(pS)YPD(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R236 to D242; In the region between the PB1 domain and the protein kinase catalytic domain
Production Method: Corresponds to amino acid residues R236 to D242; In the region between the PB1 domain and the protein kinase catalytic domain
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 66-74 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HeLa cells.
Related Product 1: MEKK2-pS239 blocking peptide
Related Product 2: MEKK2-1 pan-specific antibody (Cat. No.: AB-NK108-3)
Related Product 3: MEKK2-2 pan-specific antibody (Cat. No.: AB-NK108-4)
Related Product 4: MEKK2-3 pan-specific antibody (Cat. No.: AB-NK108-5)
Related Product 5: MEKK6-2 pan-specific antibody (Cat. No.: AB-NK225-2)
Related Product 6: MEKK6-3 pan-specific antibody (Cat. No.: AB-NK225-3)
Scientific Background: MEKK2 (MAP3K2) is a protein-serine/threonine kinase of the STE group and STE11 family. It is required for cytokine production in cultured mast cells. The protein is one of the kinases that regulate different steps of the caveolar cycle. It activated by phosphorylation on T524. Methylation of the protein increases MAP kinase signalling and promotes Ras-driven carcinomas.
