Product Name:
CSF1R-pY699
Product Number:
ab-pk587
Target Full Name: Macrophage colony-stimulating factor 1 receptor
Target Alias: CD115; C-fms; Colony stimulating factor 1 receptor; CSF-1-R; CSFMR; FMS; Fms proto-oncogene; Kinase CSFR; Macrophage colony stimulating factor I receptor precursor; M-CSFR; FIM2; FIM2; CCDS4302.1; A2VDG3; Q6LEI2; ENSG00000182578
Product Type Specific: CSF1R protein kinase phosphosite-specific antibody
Antibody Code: PK587
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y699
Protein UniProt: P07333
Protein SigNET: CSF1R
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CSF1R (Fms) sequence peptide Cat. No.: PE-04AKD95
Antibody Immunogen Sequence: GVD(pY)KNI(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G696 to I702; in the kinase catalytic domain. Y699 phosphorylation induces binding to PIK3CB and Grb2. This is the major in vivo phosphorylation site in CSF1R (≥36 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). CSF1R is known to be phosphorylated at this site in vitro by CSF1R.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in A431 and Jurkat cells. Very strong immunoreactivity with immunogen peptide on dot blots. Medium-strong immunoreactivity with recombinant human CDF1R on protein dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human (The amino acid residues prior to the phosphosite are not well conserved).
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 120-130 kDa.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: Medium immunoreactivity on protein dot blots with recombinant human Ftt3 and Kit. No significant cross-reactive proteins detected in Jurkat and HeLa cells. Strongly cross-reactive 45 and 50 kDa proteins were detected in A431 cells, and a 75 kDa cross-reactive protein was evident in sea star oocytes.
Related Product 1: Fms (696-702) pY699 antibody blocking peptide (Cat. No.: PE-04AKD95)
Related Product 2: CSF1R expression antibody (Cat. No.: AB-NK234-3)
Related Product 3: CSF1R expression antibody (Cat. No.: AB-NK234-2)
Related Product 4: CSF1R expression antibody (Cat. No.: AB-NK234-4)
Related Product 5: CSF1R S807+Y809 phosphorylation antibody (Cat. No.: AB-PK586)
Related Product 6: CSF1R Y809 phosphorylation antibody (Cat. No.: AB-PK588)
Scientific Background: CSF1R (Fms; CD115) is a member of the TK group of protein kinases in the PDGFR family. It acts as a membrane receptor for macrophage colony-stimulating factor 1 (CSF1) and IL34. It functions in the regulation of cell survival, proliferation, and differentiation in hematopoietic precursor cells as well as osteoclasts for bone metabolism. Activation of CSF1R triggers signalling pathways, including Ras/Erk and PI3K, that are crucial for myeloid cell development. Orthologues of CSF1R are highly conserved in mammals and birds. CSF1R contains a so-called kinase insert domain and is a member of the CSF1/PDGF receptor family of tyrosine-protein kinases. CSF1R is predominantly localized to the plasma membrane of myeloid lineage cells, particularly macrophages and microglia in the brain, and osteoclasts in bone. However, this kinase is highly expressed and widely distributed in most tested human tissues except in the brain and spinal cord, where it is more moderately expressed. Mutations in CSF1R have been associated with providing sustained signals for cell growth and a predisposition to myeloid malignancy. Abnormalities in CSF1R signaling are associated with hereditary diffuse leukoencephalopathy with spheroids (a neurodegenerative disease) and tumor-associated macrophages (TAMs). CSF1R has been linked with the development of acute myeloid leukemia, chronic myelomonocytic leukemia, myelodysplasia, hepatocellular carcinomas, breast cancer, and lung squamous cell carcinomas (LSCC). This description may include information annotated by UniProt and/or Google AI.