Product Name:
TrpC7
Product Number:
ab-nn351-1
Target Full Name: Short transient receptor potential channel 7
Target Alias: TRP7; KNP3; TRPM2; transient receptor potential cation channel subfamily C member 7
Product Type Specific: TRPC7 calcium channel pan-specific antibody
Antibody Code: NN351-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9HCX4
Protein SigNET: TrpC7
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Synthetic peptide patterned after human TRPC7 sequence
Antibody Immunogen Sequence: FGKNLNKDHLRVNKGKDI
Antibody Immunogen Description: Corresponds to amino acid residues F845-I862.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Medium-high potency. Detects a ~100 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-343 was sufficient for detection of TrpC7 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Medium-high
Related Product 1: TrpC5 pan-specific antibody (Cat. No.: AB-NN350-1)
Related Product 2: TrpM7 pan-specific antibody (Cat. No.: AB-NK305-1)
Related Product 3: TrpV3 pan-specific antibody (Cat. No.: AB-NN353-1)
Scientific Background: Transient receptor potential cation channel, subfamily C, member 7, also known as TRPC7, is a non-selective cation channel that is directly activated by DAG. TrpC7 shows constitutive activity and susceptibility to negative regulation by extracellular Ca2+. Because of this, TrpC7 plays an important role in the Ca2+ signalling pathway (1). TrpC7 is also expressed abundantly in the heart, and combined with its ability to act as a Ca2+ channel, TrpC7 might contribute to the process of heart failure (2). This description may include information annotated by UniProt and/or Google AI.