Product Name:
GRP78
Product Number:
ab-nn048-8
Target Full Name: 78 kDa glucose-regulated protein
Target Alias: BIP; Grp78; HSPA5; MIF2; immunoglobulin heavy chain binding protein
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN048-8
Antibody Target Type: Pan-specific
Protein UniProt: P11021
Protein SigNET: GRP78
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: His-tagged rat GRP78 (Uniprot ID P06761)
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~78 kDa protein in cell and tissue lysates by Western blotting. Weak detection in human tissues.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SPC-167was sufficient for detection of Grp78 in 10 µg of rat brain cell lysate by colorimetric immunoblot analysis using Goat anti -rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Medium-low.
Antibody Cross Reactivity: Detects many other strong cross-reactive proteins of diverse mass in mouse brain lysates.
Related Product 1: GRP78 (Bip) expression antibody (Cat. No.: AB-NN048-2)
Related Product 2: GRP78 expression antibody (Cat. No.: AB-NN048-3)
Related Product 3: GRP78 expression antibody (Cat. No.: AB-NN048-4)
Related Product 4: GRP78 expression antibody (Cat. No.: AB-NN048-5)
Related Product 5: GRP78 expression antibody (Cat. No.: AB-NN048-6)
Related Product 6: GRP78 (Bip) expression antibody (Cat. No.: AB-NN048-7)
Scientific Background: GRP78 (Glucose-Regulated Protein 78) is a ubiquitously expressed, 78-kDa glucose regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. It binds to hydrophobic patches on unfolded proteins to prevent aggregation and assist in folding. It features an N-terminal ATPase binding domain and a C-terminal substrate-binding domain. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are altered in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumour drugs (6, 7). While primarily in the ER, stress can trigger its translocation to the cell surface, where it acts as a receptor influencing cancer progression and cell signalling. It is upregulated in many cancers (promoting growth and angiogenesis) and neurodegenerative diseases like Alzheimer’s and Parkinson’s. This description may include information annotated by UniProt and/or Google AI.