Product Name:
ERK2-1
Product Number:
ab-nk055-7
Target Full Name: Extracellular regulated protein-serine kinase 2 (p42 MAP kinase)
Target Alias: ERK; ERK-2; ERT1; Extracellular signal regulated kinase 2; Kinase ERK2; MAP kinase 2; MAP kinase isoform p42; MAPK; MAPK 2; PRKM2; p41mapk; CCDS13795.1; ENSG00000100030
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK056-2
Antibody Target Type: Pan-specific
Protein UniProt: P28482
Protein SigNET: P28482
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ERK2 (MAPK1) sequence peptide Cat. No.: PE-01AXE99
Antibody Immunogen Sequence: CYLLSLPHKNKVPWNR
Antibody Immunogen Description: Corresponds to amino acid residues Y263 to R277; Pre-kinase last alpha-chain
Production Method: Corresponds to amino acid residues Y263 to R277; Pre-kinase last alpha-chain
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-44 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: Very weak immunoreactivity on protein dot blots with recombinant human ERK2 and ERK5.
Related Product 1: ERK2-1 blocking peptide
Related Product 2: ERK2-2 pan-specific antibody (Cat. No.: AB-NK056-3)
Related Product 3: ERK2-3 pan-specific antibody (Cat. No.: AB-NK056-4)
Related Product 4: ERK2-pT185+pY187 phosphosite-specific antibody (Cat. No.: AB-PK622)
Related Product 5: ERK1-pT202+pY204 phosphosite-specific antibody (Cat. No.: AB-PK621)
Related Product 6: ERK1-pY204 phosphosite-specific antibody (Cat. No.: AB-PK864)
Related Product 7: ERK1-pT207 phosphosite-specific antibody (Cat. No.: AB-PK865)
Related Product 8: ERKSelectideA - ERK1 (MAPK3) protein kinase substrate peptide
Related Product 9: ERKSelectideB - ERK1 (MAPK3) protein kinase substrate peptide
Related Product 10: ERKSubtide - ERK1 (MAPK3) protein kinase substrate peptide
Scientific Background: ERK2 (MAPK1) is a protein-serine/threonine kinase of the CMGC group and MAPK family. It is involved in the MAP Kinase signalling pathway. The MAP kinase pathway can mediate cell adhesion, growth, survival, differentiation, meiosis, mitosis, and other post-mitotic functions. Phosphorylation of T185 and Y187 by MEK1 an MEK2 increases its phosphotransferase activity. It phosphorylates many diffrent transcription factors, such as ELK1. It acts as transcriptional repressor by binding directly to DNA. It is essential for cyclin D1 induction. MAPK1 phosphorylates BCL2, which contributes to cell survival, the suppression of the apoptotic effect of BAD and up-regulation of the antiapoptotic protein MCL-1. It regulates accumulation of p53 during DNA damage response. ERK2 appears to be a tumour requiring protein (TRP). The active form of the protein kinase normally acts to promote tumour cell proliferation. ERK2 has been linked with the Ewing's Family of tumours, which are rare cancers that are similar to medullablastoma and cerebral primitive neuroectodermal tumours. It is constitutively active in many human tumours, primarily due to altered Ras, Raf, EGFR or other upstream oncoproteins.
